Impact on the productivity of preparation on rhizobial inoculant carriers
Keywords:
Carrier material, Inoculants, Biofertilizers, Strain, Biomass, Nodulation
Abstract
Selection of a suitable carrier material for rhizobial inoculants is essential for biofertilizers production. Locally available wastes or by-products as carrier material will increase the cost effectiveness of the inoculants preparation. Here, were evaluated four such waste materials from local ground viz. charcoal, saw dust, garden soil and sugarcane bagasse with carrier based inoculums (108 viable cells/ml) and kept at room temperature (30 ± 20C). The colony forming unit (CFU) count of each strain in different carriers was monitored every month. The charcoal, garden soil and saw dust resulted to allow a better survival of the inoculums. The viable counts in charcoal, soil, saw dust and sugarcane bagasse after 240 days of storage was recorded as 107, 106, 105 and 103 for MPR8 and 107, 105, 105 and 103 for TFR3 strains respectively. The effects of storage of carrier on plant productivity showed better plant biomass accumulation and nodulation in cases of charcoal, sawdust and garden soil. However it was insignificant with the sugarcane bagasse based inoculants.
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References
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8. Somasegaran P, Hoben HJ, Burton JC. A medium scale fermentor for mass culture of rhizobia. World J Microbiol Biotechnol. 1992; 8: 335-336.
9. Deschodt CC, Strijdom, BW. Suitability of a coal-bentonite base as carrier of rhizobia in inoculants. Phytophylectica. 1976; 8: 1-6.
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28. Paczkowski ME, Berryhill DL. Survival of Rhizobium phaseoli in coal based inoculants. Environ Microbiol. 1979; 38: 612-615.
29. Faizah AW, Broughton, WJ, John CK. Rhizobia in tropical legumes-x. Growth in coir-dust-soil compost. Soil Biol Biochem. 1980; 12: 211-218.
30. Calabi-Floody M, Medina J, Rumpel C, Condron LM, Hernandez M, Dumont M, Luz Mora M. Smart Fertilizers as a Strategy for Sustainable Agriculture. Adv Agron. 2018; 147: 119-157.
31. Rebah B, Faouzi Prévost D, Yezza A, Tyagi R. Agro-industrial waste materials and wastewater sludge for rhizobial inoculant production: A review. Biores Technol. 2008; 98: 3535-46.
32. Tilak KV, Jauhri BR, KS, Saxena AK. Rhizobium fertiliser technology for legumes. I.A.R.I., New Delhi (India) 1966; 1-32.
33. Bashan Y. Inoculants of plant growth promoting bacteria for use in agriculture. Biotech Adv. 1998; 16: 729-770.
34. Arora NK, Kumar V, Maheshwari DK. Constraints, development and future of the bio-inoculants with special reference to rhizobial inculants. In: Innovative Approaches in Microbiology, Maheshwari DK, Dubey RC eds. Bishen Singh Mahendra Pal Singh Publishers, Dehra Dun, India. 2001; 241-254.
35. Lockhead AG, Thexton RH. Growth and survival of bacteria in pea. Can J Res. 1947; 25: 1-3.
36. Vincent JM. In: Nutrition of the Legume. Hallsworth EG, ed. Butterworths, London. 1958; 108-123.
37. Mc Leod RW, Roughley RS. Freeze- dried cultures as commercial legume inoculant. Aust J Agric Anim Husb. 1961; 1: 29-33.
38. Kremer RJ, Peterson HL. Effects of carrier and temperature on survival of Rhizobium sp. in legume inocula: development of an improved type of inoculant. Appl Environ Microbiol. 1983; 45: 1790-1794.
39. Chanaseni C, Kongngoen S. Extension programmes to promote rhizobial inoculants for soybean and groundnut in Thailand. Can J Microbial. 1992; 38: 572-588.
40. Głodowska M, Schwingharner T, Barry H, Smith D. Biochar Based Inoculants Improve Soybean Growth and Nodulation. Agric Sci. 2017; 08: 1048-1064.
41. Dazzo A. Santamaria C, Rodriguez-Navarro DN, Comacho M, Oriv R, Temperano, F. Perlite as a carrier for bacterial inoculants. Soil Biol Biochem. 2000; 32: 567-572.
42. Ciafardini G, Marnelli G, Missich R. Soil biomass of B. japonicum via irrigation water. Can J Microbiol. 1992; 38: 584-587.
43. Arora NK, Verma M, Mishra J. Rhizobial bioformulations: Past, Present and Future. Springer. 2017; 69-99.
2. Egamberdieva D, Ma H, Alimov J, Reckling M, Wirth S, Bellingrath-Kimura SD. Response of Soybean to Hydrochar-Based Rhizobium Inoculation in Loamy Sandy Soil. Microorganisms. 2020; 8(11): 1674.
3. Khatri N, Tyagi S. Influences of natural and anthropogenic factors on surface and groundwater quality in rural and urban areas. Front Life Sci. 2015; 2; 8(1): 23-39.
4. Gomare KS, Mese M, Shetkar Y. Isolation of Rhizobium and cost effective production of biofertilizer. Ind J Life Sci. 2013; 2(2): 49-53.
5. Hellriegel H, Wilfarth H. Untersuchungen über die stickstoffnahrung der Gramineen und Leguminosen. Belageheft zu der zeitschrift des zeitschrift des vereins Rübenzucker-Industrie Deutschen, Reiches. 1888; 1: 234.
6. Fred EB, Baldwin IL, McCoy E, Root nodule bacteria and leguminous plants. University of Wisconsin studies in Science No. 5. 1932.
7. Goyal SK. Cynobacterial inoculants for sustainability in rice cultivation. In: National Seminar on Biotechnology-New Trends & Prospects. Gurukul Kangri University, Hardwar. 1966; 22.
8. Somasegaran P, Hoben HJ, Burton JC. A medium scale fermentor for mass culture of rhizobia. World J Microbiol Biotechnol. 1992; 8: 335-336.
9. Deschodt CC, Strijdom, BW. Suitability of a coal-bentonite base as carrier of rhizobia in inoculants. Phytophylectica. 1976; 8: 1-6.
10. Parker FE, Vincent JM. Sterilization of peat by γ-irradiation. Plant Soil. 1981; 61: 285-293.
11. Roughley RJ. Some factors influencing the growth and survival of root nodule bacteria in peat culture. J Appl Bacteriol. 1968; 31: 259-265.
12. Bajpai PD, Gupta BR, Ram B. Studies on survival of Rhizobium leguminosarum in two carries as affected by moisture and temperature conditions. Ind J Agric Res. 1979; 112: 39-43.
13. Pastor BR, Sánchez-Cañizares C, James EK, González AF. Formulation of a Highly Effective Inoculant for Common Bean Based on an Autochthonous Elite Strain of Rhizobium leguminosarum bv. phaseoli, and Genomic-Based Insights Into Its Agronomic Performance. Front Microbiol, 2019; 10: 2724.
14. Kloepper JW, Lifshitz R, Zablotowicz RM. Free living bacteria inocula enhancing crop productivity. Trends Biotechnol. 1989; 7: 39-44.
15. Glick BR. The enhancement of plant growth by free living bacteria. Can J Microbiol. 1995; 41: 109-117.
16. Fages J. An industrial view of Azospirillum inoculants: formulation and application technology. Symbiosis. 1992; 13: 15-26.
17. Somasegaran P, Hoben H. Methods in Legume-Rhizobium Technology. University of Hawaii, NiFTAL Project. 1985.
18. Gomez KA, Gomez AA. Statistical Procedures for Agricultural Research, A Wiley-Interscience Publication, John Wiley and Sons, New York, USA. 1984.
19. Sparrow SD, Ham DE. Survival of Rhizobium phaseoli in six carrier materials. Agro J. 1983; 75: 181-184.
20. Beck DP. Suitability of charcoal amended mineral soil as carrier for Rhizobium inoculants. Soil Biol Biochem. 1991; 23(1): 41-44.
21. Nair KS, Ramaswami PP, Perumal, R. Survival of rhizobia in storage. Madras Agric J. 1970; 57: 63-66.
22. Arora NK, Ekta Khare R, Niranjan R, Maheshwari DK. Sawdust as a superior carrier for production of multipurpose bio-inoculant using plant growth promoting rhizobial and Pseudomonad strains and their impact on productivity of Trifolium repens. Curr Sci. 2008; 90-94.
23. Roughlev RJ, Vincent JM. Growth and Survival of Rhizobium spp. in Peat Culture. J Appl Microbiol. 1967; 30: 362-376.
24. Chao WL, Alexander M. Mineral Soils as carrier for Rhizobium inoculants. Appl Env Microbiol. 1984; 47: 94-97.
25. Yami KD. Improvement of grain legumes by Rhizobium inoculation. In: Papers Presented at the first review working group meeting on Bio-fertilizer technology. Kathmandu Nepal, 1987.
26. Muniruzzaman S, Khan SI. Suitability of some local agro-industrial wastes as carrier materials for Rhizobium sp. infecting Sesbania bispinosa. World J Microbiol. Biotechnol. 1992; 8: 329-330.
27. Deschodt CC, Strijdom BW. Carriers of rhizobia and the effects of prior treatment on the survival on rhizobia. In: Symbiotic Nitrogen Fixation in Plants. Nutman PS, ed. International Biological Programme no. 7. Cambridge, UK. 1976.
28. Paczkowski ME, Berryhill DL. Survival of Rhizobium phaseoli in coal based inoculants. Environ Microbiol. 1979; 38: 612-615.
29. Faizah AW, Broughton, WJ, John CK. Rhizobia in tropical legumes-x. Growth in coir-dust-soil compost. Soil Biol Biochem. 1980; 12: 211-218.
30. Calabi-Floody M, Medina J, Rumpel C, Condron LM, Hernandez M, Dumont M, Luz Mora M. Smart Fertilizers as a Strategy for Sustainable Agriculture. Adv Agron. 2018; 147: 119-157.
31. Rebah B, Faouzi Prévost D, Yezza A, Tyagi R. Agro-industrial waste materials and wastewater sludge for rhizobial inoculant production: A review. Biores Technol. 2008; 98: 3535-46.
32. Tilak KV, Jauhri BR, KS, Saxena AK. Rhizobium fertiliser technology for legumes. I.A.R.I., New Delhi (India) 1966; 1-32.
33. Bashan Y. Inoculants of plant growth promoting bacteria for use in agriculture. Biotech Adv. 1998; 16: 729-770.
34. Arora NK, Kumar V, Maheshwari DK. Constraints, development and future of the bio-inoculants with special reference to rhizobial inculants. In: Innovative Approaches in Microbiology, Maheshwari DK, Dubey RC eds. Bishen Singh Mahendra Pal Singh Publishers, Dehra Dun, India. 2001; 241-254.
35. Lockhead AG, Thexton RH. Growth and survival of bacteria in pea. Can J Res. 1947; 25: 1-3.
36. Vincent JM. In: Nutrition of the Legume. Hallsworth EG, ed. Butterworths, London. 1958; 108-123.
37. Mc Leod RW, Roughley RS. Freeze- dried cultures as commercial legume inoculant. Aust J Agric Anim Husb. 1961; 1: 29-33.
38. Kremer RJ, Peterson HL. Effects of carrier and temperature on survival of Rhizobium sp. in legume inocula: development of an improved type of inoculant. Appl Environ Microbiol. 1983; 45: 1790-1794.
39. Chanaseni C, Kongngoen S. Extension programmes to promote rhizobial inoculants for soybean and groundnut in Thailand. Can J Microbial. 1992; 38: 572-588.
40. Głodowska M, Schwingharner T, Barry H, Smith D. Biochar Based Inoculants Improve Soybean Growth and Nodulation. Agric Sci. 2017; 08: 1048-1064.
41. Dazzo A. Santamaria C, Rodriguez-Navarro DN, Comacho M, Oriv R, Temperano, F. Perlite as a carrier for bacterial inoculants. Soil Biol Biochem. 2000; 32: 567-572.
42. Ciafardini G, Marnelli G, Missich R. Soil biomass of B. japonicum via irrigation water. Can J Microbiol. 1992; 38: 584-587.
43. Arora NK, Verma M, Mishra J. Rhizobial bioformulations: Past, Present and Future. Springer. 2017; 69-99.
Published
2021-03-27
How to Cite
(1)
Paudyal, S.; Das, B.; Paudel, V.; Paudel, N. Impact on the Productivity of Preparation on Rhizobial Inoculant Carriers. European Journal of Biological Research 2021, 11, 242-250.
Section
Research Articles
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