European Journal of Biological Research http://journals.tmkarpinski.com/index.php/ejbr <p><strong>ISSN: 2449-8955</strong><br><strong><a title="MNiSW points: 11" href="http://www.nauka.gov.pl/g2/oryginal/2016_12/c5c2fcb0c283a9eb3d1081020fd3178c.pdf" target="_blank" rel="noopener">MNiSW points 2016: 11<br></a></strong><strong><a title="ICV: 100.00" href="http://journals.indexcopernicus.com/European+Journal+of,p24783440,3.html" target="_blank" rel="noopener">ICV 2017: 100.00</a></strong><br><strong>Acceptance rate: 2016 - 64%, 2017 - 56%</strong><br><strong>Google-based IF (2017): 0.586&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; h-index: 11</strong></p> JBródka Publisher en-US European Journal of Biological Research 2449-8955 Efficacy of octenidine against Pseudomonas aeruginosa strains http://journals.tmkarpinski.com/index.php/ejbr/article/view/207 <p><em>Pseudomonas aeruginosa</em> is a Gram-negative bacterium causing skin and soft tissue infections, complicated urinary tract infections, blood infections, and nosocomial (hospital-acquired) infections. One of the most often used antiseptics in the skin and soft tissue infections is octenidine. The aim of this study was an evaluation of octenidine activity against strains of <em>P. aeruginosa.</em> Additionally, were compared two staining methods (TTC and MTT) for bacterial growth detection. The study involved eight strains of <em>P. aeruginosa</em>. In order to determine the minimum inhibitory concentration (MIC) of octenidine, the microdilution method was used. For bacterial growth detection was used staining method with 2,3,5-triphenyl-tetrazolium chloride (TTC) and with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). In the study has been demonstrated the excellent activity of octenidine against all strains of <em>Pseudomonas aeruginosa</em>. For all tested strains, MICs of octenidine were 0.000195% or 0.00039%, what is equivalent to 1.95 µg/ml and 3.9 µg/ml, respectively. In the study, test with MTT for three strains was more sensitive than a test with TTC. Concluding, octenidine is an antiseptic with high efficacy against <em>Pseudomonas aeruginosa </em>strains. Simultaneously, it was stated that a test with MTT is more sensitive than study with TTC.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.3339499">http://dx.doi.org/10.5281/zenodo.3339499</a></strong></p> Tomasz M. Karpiński ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-06-18 2019-06-18 9 3 135 140