European Journal of Biological Research http://journals.tmkarpinski.com/index.php/ejbr <p><strong>ISSN: 2449-8955</strong><br><strong><a title="MNiSW points: 11" href="http://www.nauka.gov.pl/g2/oryginal/2016_12/c5c2fcb0c283a9eb3d1081020fd3178c.pdf" target="_blank" rel="noopener">MNiSW points 2016: 11<br></a></strong><strong><a title="ICV: 100.00" href="http://journals.indexcopernicus.com/European+Journal+of,p24783440,3.html" target="_blank" rel="noopener">ICV 2017: 100.00</a></strong><br><strong>Acceptance rate: 2016 - 64%, 2017 - 56%</strong><br><strong>Google-based IF (2017): 0.586&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; h-index: 11</strong></p> TMKarpinski Publisher en-US European Journal of Biological Research 2449-8955 Therapeutic effect of fractionated by ultrafiltration red beetroot (Beta vulgaris L.) juice in rats with food-induced fatty liver http://journals.tmkarpinski.com/index.php/ejbr/article/view/108 <p>The prevalence of non-alcoholic fatty liver disease (NAFLD), being a component of metabolic syndrome, has increased (15-27%) in the industrialized world. The deep mechanism of this pathology is not clear, but it is multifactorial. There is a huge amount of food supplements and medicines with hepatoprotective effect on the market, but the NAFLD problem is far from being resolved. Hepatoprotective products have to provide wide spectra of biological effects, including antioxidant, hypolipidemic, anti-inflammatory action. It is peculiar to natural compounds, including red beetroot juice, which is well known to most of the population. This is important in view of the high prevalence of NAFLD. The aim of this study is to evaluate the curative effect of fractionated by ultrafiltration red beetroot juice in rats with food-induced liver steatosis.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.2541075">http://dx.doi.org/10.5281/zenodo.2541075</a></strong></p> Dmitry Babarykin Galina Smirnova Jurijs Markovs Svetlana Vasiljeva Natalija Basova Raimonds Simanis Ludmila Viksna ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-01-15 2019-01-15 9 1 1 9 Phenolics content, antiproliferative and antioxidant activities of Algerian Malva sylvestris http://journals.tmkarpinski.com/index.php/ejbr/article/view/102 <p>Due to its expected low toxicity to human use, more attention is given worldwide to antioxidants of natural sources. Therefore, the extraction of the total phenolic compounds contained in the leaves of<em> Malva sylvestris</em> and the analysis of the polyphenols, flavonoids and tannins contents were carried out. The antioxidant activity of the hydro-methanolic extract of <em>Malva sylvestris </em>was investigated employing various established <em>in vitro</em> systems including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, the reduction of hydrogen peroxide and the ferric reducing power assay. The antiproliferative activity of plant extract was tested against three tumor cell lines: MCF-7, Hep2 and WEHI using 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) assay. Preliminary screening indicated the presence of substances with large therapeutic values: an important content of polyphenols, flavonoids and tannins was detected in the tested extract. Our data showed that the extract exhibited high antioxidant properties, which were demonstrated by its ability to scavenge 76.11% of DPPH free radicals, and the elimination of 69.97% of hydrogen peroxide at a concentration of 125 µg/ml. In addition, the plant extract showed strong ferric reducing power which was a function of the sample concentration. For the antiproliferative activity, the results demonstrated that the plant extract significantly inhibited tumor cell growth and colony formation in a concentration-dependent manner. The toxicity percentage of extract at 125 µg/ml on MCF-7, Hep2 and WEHI was found in the order of 45.20%, 62.62% and 82.04%, respectively.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.2545914">http://dx.doi.org/10.5281/zenodo.2545914</a></strong></p> Hanane Messaoud Boutennoun Lilia Boussoufe Mohamed Kebieche Khaled Al-Qaoud Khodir Madani ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-01-21 2019-01-21 9 1 10 19 Production and purification of extreme xylanase from Aspergillus flavus AUMC 10331 in sub-merged fermentation http://journals.tmkarpinski.com/index.php/ejbr/article/view/111 <p>Xylan, extracted from oat spelts in a previous work, was assayed by HPLC and used as carbon source for the production of xylanase from <em>Aspergillus flavus </em>AUMC 10331. The produced xylanase was purified using ion exchange resin (IR-120 EP) and gel filtration column of Sephadex G-75 and Sephadex G-100. The purified xylanase showed total activity of 5.5 IU/ml and specific activity of 687.5 IU/mg, and the enzyme purified 156.75 fold with 4.43 % yield. The highest activity at pH 7.0 and 10.5 indicating two xylanases with the most interesting one with a maximum activity at pH 10.5 and 65 °C. The enzyme activity was greatly stimulated by 5 mM of FeSO<sub>4</sub> and CuSO<sub>4</sub>, while slightly inhibited by other metal ions. K<sub>m</sub> and V<sub>max </sub>were determined as 8.36 mg/ml and 172.4 IU/min respectively.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.2586103">http://dx.doi.org/10.5281/zenodo.2586103</a></strong></p> AH Moubasher Mady Ismail Ramadan Mohamed Osama Al-Bedak ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-03-06 2019-03-06 9 1 20 28 The oldest known site of the Roman snail (Helix pomatia L.) in the Ślęża Mountain (SW Poland) http://journals.tmkarpinski.com/index.php/ejbr/article/view/121 <p>Study on the distribution of the Roman snail in the Ślęża Massif was carried out in 2016. The species was recorded at the foot of the Ślęża and Radunia Mountains, on their slopes (to the 300 m a.s.l.) and at the top of the Ślęża Mountain. The slopes of the Ślęża and Radunia Mountains are covered with spruce and beech forests without undergrowth which is unfavorable for this snail. Therefore, we suspect that the population of the species on the top of the Ślęża Mountain is an isolated and the oldest population of <em>Helix pomatia </em>recorded in Poland.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.2586109">http://dx.doi.org/10.5281/zenodo.2586109</a></strong></p> Jerzy Błoszyk Szymon Konwerski Joanna Gogol Agnieszka Napierała ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-03-06 2019-03-06 9 1 29 33