Antidiabetic potential of mucilage fraction extracted from Astragalus gyzensis seeds

  • Aicha Tedjani Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla 30000, Algeria
  • Zakaria Boual Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla 30000, Algeria
  • Mohamed Didi Ould El Hadj Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla 30000, Algeria
  • Touhami Lanez Laboratory of Valorisation and Technology of Sahara Resources (VTRS) University Echahid hamma lakhdar, El-Oued 39000, Algeria
  • Hakim Belkhalfa Scientific and Technical Research Center in Physicochemical Analysis, Tipaza 42000, Algeria
  • Zainab El Alaoui-Talibi Faculty of Sciences and Techniques, University of Cadi Ayyad, Marrakech 40000, Morocco
  • Cherkaoui El Modafar Faculty of Sciences and Techniques, University of Cadi Ayyad, Marrakech 40000, Morocco
  • Slim Abdelkafi Laboratory of Enzymatic Engineering and Microbiology, Algae Biotechnology Team, National Engineering School of Sfax, Sfax University, Sfax 3038, Tunisia
  • Imen Fendri Laboratory of Plant Biotechnology Applied to the Improvement of Plants, Faculty of Sciences, Sfax University, Sfax 3038, Tunisia
  • Didier Le Cerf Department of Chemistry, University of Rouen Normandie, INSA Rouen, CNRS, PBS, 76000 Rouen, France
  • Pascal Dubessay Department of Chemistry, University of Rouen Normandie, INSA Rouen, CNRS, PBS, 76000 Rouen, France
  • Cédric Delattre Institute of Pascal, University of Clermont Auvergne, CNRS, Clermont Auvergne INP, 63000 Clermont-Ferrand, France
  • Pierre Guillaume Institute of Pascal, University of Clermont Auvergne, CNRS, Clermont Auvergne INP, 63000 Clermont-Ferrand, France
  • Philippe Michaud Institute of Pascal, University of Clermont Auvergne, CNRS, Clermont Auvergne INP, 63000 Clermont-Ferrand, France
Keywords: Astragalus gyzensis Bunge, Mucilage, Antidiabetic, Galactomannans, Dietary supplement fiber

Abstract

The objective of the current work is to extract a new mucilage fraction from Astragalus gyzensis Bunge. seeds, which are collected from the El-Oued province (septentrional Algerian Sahara) and evaluated for their antidiabetic potential. The mucilage fraction is obtained using hot water extraction followed by alcoholic precipitation of polysaccharides by cold ethanol (96%). The primary investigation was performed by describing the main structural features of the extract through colorimetric assays, Fourier-transform infrared spectroscopy and thin-layer chromatography analysis using two systems. Biological activity was also monitored by antidiabetic activity by testing the inhibition of α-amylase and α-glucosidase enzymes in vitro. The extraction yield was 20.69%. The chemical composition mainly consisted of 78.60±0.29% carbohydrates, among them 63.92±0.67% neutral sugar, 15.78±0.76% uronic acid, 8.08±0.04% proteins and 2.57±0.05% phenolic compounds. The results obtained by thin-layer chromatography analysis showed the dominance of mannose and galactose. Fourier-transform infrared spectrum showed characteristic bands expected galactomannans. The investigations highlighted the antihyperglycemic effect in a dose-dependent manner by the inhibition of the α-amylase enzyme (IC50=0.8±0.005 mg/mL). These factors make it suitable for the industrial application of dietary supplement fiber made for diabetic individuals.

DOI: http://dx.doi.org/10.5281/zenodo.7618530

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Published
2023-02-07
How to Cite
(1)
Tedjani, A.; Boual, Z.; Ould El Hadj, M. D.; Lanez, T.; Belkhalfa, H.; El Alaoui-Talibi, Z.; El Modafar, C.; Abdelkafi, S.; Fendri, I.; Le Cerf, D.; Dubessay, P.; Delattre, C.; Guillaume, P.; Michaud, P. Antidiabetic Potential of Mucilage Fraction Extracted from Astragalus Gyzensis Seeds. European Journal of Biological Research 2023, 13, 18-30.
Issue
Vol 13 No 1 (2023): January-March 2023
Section
Research Articles
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