Blocking IgE with L-glutamic acid analogs as an alternative approach to allergy treatment

  • Débora Mothé de Campos-Mesquita Laboratory of Chemistry and Function of Proteins and Peptides, Universidade Estadual do Norte Fluminense (UENF) - Darcy Ribeiro, Campos dos Goytacazes, RJ 2000, Brazil
  • Giliane Silva de Souza Laboratory Biology of Recognition, UENF, Campos dos Goytacazes, RJ 2000, Brazil
  • Marinete Pinheiro Carrera Laboratory of Animal Morphology and Pathology, UENF, Campos dos Goytacazes, RJ 2000, Brazil
  • Arthur Giraldi-Guimarães Laboratory of Cell and Tissue Biology, UENF, Campos dos Goytacazes, RJ 2000, Brazil
  • Olga Lima Tavares Machado Laboratory of Chemistry and Function of Proteins and Peptides, Universidade Estadual do Norte Fluminense (UENF) - Darcy Ribeiro, Campos dos Goytacazes, RJ 2000, Brazil
Keywords: Allergy treatment, 2S Albumin, IgE blocker


IgE-mediated allergic diseases have increased in the last decades. The most prevalent allergens from these seeds are Ric c1 and Ric c3, isoforms of 2S albumin. These allergenic proteins cross-react with allergens from peanut, shrimp, fish, corn, gramineous, house dust, and tobacco. The usual allergy treatment employs antihistaminic, immunotherapies and, omalizumab (Xolair)-based anti-IgE therapy. However, antihistaminics relieve symptoms, and the high cost of omalizumab limits its use for continuous treatment. We propose an alternative immunotherapeutic approach, denoted “IgE-blockage” by L-glutamic acid or modified-glutamic acid. Six compounds, D-glutamic acid, L-glutamic acid, N-methyl-L-glutamic acid, N-acetyl-L-glutamic acid, N-(4-nitrobenzoyl)-L-glutamic acid, and N-carbamyl-L-glutamic, were tested as a blocker. To evaluate motor coordination and the sedative/hypnotic activity of L-glutamic acid, a rota-rod test and a thiopental sodium-induced sleeping test were used. The compounds, L-glutamic acid and L-nitrobenzoyl glutamic acid, were the most active compounds to block the interaction of castor allergens with IgE. These compounds also prevent cross-responses with allergens from food sources and inhalants that cross-react with them. In the sleeping test, the groups that received L-glutamic acid at doses of 10 and 30 mg/kg had a sleeping time similar to the vehicle control group. No changes in the animals' behavior were observed and there was no difference between the L-glutamic acid groups and the vehicle control groups in the rota-rod test. L-glutamic acid and L-nitrobenzoyl glutamic acid can used as IgE blocker to prevent allergic diseases.



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1. Holloway JW, Yang IA, Holgate ST, Sci F. Genetics of allergic disease. J Allergy Clin Immunol. 2010; 125: S81-S94.
2. Yu Y, Zhang H, Wang W, Wang S. Selective adsorbent for the removal of immunoglobulin E in bronchial asthma. Artif Cells Blood Substit Biotechnol. 2008; 36: 63-73.
3. Gangwar RS, Friedman S, Seaf M, Levi-Schaffer F. Mast cells and eosinophils in allergy: Close friends or just neighbors. Eur J Pharmacol. 2016; 778: 77-83.
4. D’Amato G. Role of anti-IgE monoclonal antibody (omalizumab) in the treatment of bronchial asthma and allergic respiratory diseases. Eur J Pharmacol. 2006; 533: 302-307.
5. Wang XY, Lim-Jurado M, Prepageran N, Tantilipikorn P, Wang DY. Treatment of allergic rhinitis and urticaria: A review of the newest antihistamine drug bilastine. Ther Clin Risk Manag. 2016; 12: 585-597.
6. Ward DE, Fay BL, Adejuwon A, Han H, Ma Z. Chimeric antigen receptors based on low affinity mutants of FcϵRI Re-direct T cell specificity to cells expressing membrane IgE. Front Immunol. 2018; 9: 1-11.
7. Mahdy AM, Webster NR. Histamine and antihistamines. Anaesth Intensive Care Med. 2008; 9: 324-328.
8. Pastorello E, Pompei C, Pravettoni V, Brenna O, Farioli L, Trambaioli C, et al. Lipid transfer proteins and 2S albumins as allergens. Allergy. 2001; 56 Suppl 6: 45-47.
9. Mittag D, Batori V, Neudecker P, Wiche R, Friis EP, Ballmer-Weber BK, et al. A novel approach for investigation of specific and cross-reactive IgE epitopes on Bet v 1 and homologous food allergens in individual patients. Mol Immunol. 2006; 43: 268-278.
10. Da Silva JG, Machado OLT, Izumi C, Padovan JC, Chait BT, Mirza UA, et al. Amino acid sequence of a new 2S albumin from Ricinus communis which is part of a 29-kDa precursor protein. Arch Biochem Biophys. 1996; 336: 10-18.
11. Baumann MJ, Eggel A, Amstutz P, Stadler BM, Vogel M. DARPins against a functional IgE epitope. Immunol Lett. 2010; 133: 78-84.
12. Holgate ST, Polosa R. Treatment strategies for allergy and asthma. Nat Rev Immunol. 2008; 8: 218-230.
13. Valenta R, Karaulov A, Niederberger V, Zhernov Y, Elisyutina O, Campana R, et al. Allergen Extracts for In Vivo Diagnosis and Treatment of Allergy: Is There a Future?. J Allergy Clin Immunol Pract. 2018; 6: 1845-1855
14. 14 Lanser BJ, Wright BL, Orgel KA, Vickery BP, Fleischer DM. Current Options for the Treatment of Food Allergy. Pediatr Clin North Am 2015; 62: 1531-1549.
15. Felix SP, Mayerhoffer RO, Damatta RA, Verícimo MA, Nascimento VV, Machado OLT. Mapping IgE-binding epitopes of Ric c 1 and Ric c 3, allergens from Ricinus communis, by mast cell degranulation assay. Peptides. 2008; 29: 497-504.
16. Deus-de-Oliveira N, Felix SP, Carrielo-Gama C, Fernandes K V., DaMatta RA, Machado OLT. Identification of critical Amino acids in the IgE epitopes of Ric c 1 and Ric c 3 and the application of Glutamic acid as an IgE blocker. PLoS One. 2011; 6: 0021455.
17. Sharmen F, Mannan A, Rahman MM, Chowdhury MAU, Uddin ME, Abu Ahmed AM. Investigation of in vivo neuropharmacological effect of Alpinia nigra leaf extract. Asian Pac J Trop Biomed. 2014; 4: 137-142.
18. Ozdemir C, Kucuksezer UC, Akdis M, Akdis CA. Mechanisms of Aeroallergen Immunotherapy. Immunol Allergy Clin North Am. 2016; 36: 71-86.
19. Jayanarayanan S, Smijin S, Peeyush KT, Anju TR, Paulose CS. NMDA and AMPA receptor mediated excitotoxicity in cerebral cortex of streptozotocin induced diabetic rat: ameliorating effects of curcumin. Chem Biol Interact. 2013; 201: 39-48.
20. Woessner KM, Simon RA, Stevenson DD. Monosodium glutamate sensitivity in asthma. J Allergy Clin Immunol. 1999; 104(2 Pt 1): 305-310.
21. Obayashi Y, Nagamura Y. Does monosodium glutamate really cause headache ? a systematic review of human studies. J Headache Pain. 2016: 1-6.
22. Aswar U, Shintre S, Chepurwar S, Aswar M. Antiallergic effect of piperine on ovalbumin-induced allergic rhinitis in mice. Pharm Biol. 2015; 53: 1358-1366.
23. Thakur VD, Mengi SA. Neuropharmacological profile of Eclipta alba (Linn.) Hassk. J Ethnopharmacol. 2005; 102: 23-31.
24. Sharmen F, Mannan A, Rahman M, Chowdhury AU, Uddin ME, Ahmed MA. Investigation extract of in vivo neuropharmacological effect of Alpinia nigra leaf. Asian Pac J Trop Biomed. 2014; 4(2): 137-142.
25. Hubbard MA, Thorkildson P, Welch WH, Kozel TR. Stereo-selective binding of monoclonal antibodies to the poly-gamma-d-glutamic acid capsular antigen of Bacillus anthracis. Mol Immunol. 2013; 55: 337-344.
How to Cite
de Campos-Mesquita, D.; Silva de Souza, G.; Carrera, M.; Giraldi-Guimarães, A.; Machado, O. Blocking IgE With L-Glutamic Acid Analogs As an Alternative Approach to Allergy Treatment. European Journal of Biological Research 2023, 13, 31-40.
Research Articles