Assessment of polyphenols contents, antibacterial and antioxidant activities of Origanum majorana extracts

  • Abderrahim Benslama Department of Biochemistry and Microbiology, University of M’sila, Faculty of Sciences, M’sila, Algeria
  • Samira Daci Department of Biochemistry and Microbiology, University of M’sila, Faculty of Sciences, M’sila, Algeria
  • Larbi Zakaria Nabti Department of Biochemistry and Microbiology, University of M’sila, Faculty of Sciences, M’sila, Algeria
  • Hamdi Bendif Department of Natural and Life Sciences, Faculty of Sciences, University of M’sila, M’sila, Algeria
  • Abdenassar Harrar Department of Biochemistry and Microbiology, University of M’sila, Faculty of Sciences, M’sila, Algeria
Keywords: Antibacterial effect, Antioxidant activity, Polyphenols, Origanum majorana


The Algerian flora contains many species of vascular plants, including aromatic and medicinal plants, which can be very used for the treatment of diseases and health care. Origanum majorana is an Algerian medicinal plant used in the traditional pharmacopoeia. This work was conducted to evaluate the total polyphenolic content, antibacterial effect, and antioxidant capacity of O. majorana extracts. The extraction was carried out using the aerial parts of O. majorana with water and methanol to produce the aqueous extract (Aq.E) and the methanolic extract (Met.E). The total polyphenolic and flavonoids contents of the extracts were estimated using colorimetric method. The antibacterial effect was evaluated by the method of disc diffusion. ABTS, DPPH radical scavenging, and reducing power were used to determine the antioxidant capacity of the extracts. So, the results showed that the highest concentrations of polyphenolic amounts and flavonoids were recorded in the Met.E with values of 68.66 µg EAG/mg E and 11.71 µg EQ/mg E, respectively. Moreover, all extracts showed a good antibacterial effect against B. cereus with inhibition zones ranging from 9 to 13 mm, and moderate activity against S. aureus and P. aeruginosa. In addition, the Met.E showed the highest effect in case of DPPH and ABTS free radical (EC50=16.15±0.2 µg/ml and 19.66±0.56 µg/ml, respectively). This study demonstrated that the Met.E of O. majorana contains bioactive compounds that are related to potential biological activities, such as antioxidant and antibacterial effect.



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1. Gurib-Fakim A. Medicinal plants: Traditions of yesterday and drugs of tomorrow. Mol Asp Med. 2006; 27: 1-93.
2. Siti HN, Kamisah Y, Kamsiaha J. The role of oxidative stress, antioxidants and vascular inflammation in cardiovascular disease. Vascul Pharmacol. 2015; 71: 40-56.
3. Taylor PW, Stapleton PD, Paul-Luzio J. New ways to treat bacterial infections. Drug Discov Today. 2002; 7: 1086-1091.
4. Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009; 2(5): 270-278.
5. Scalbert A, Johnson IT, Saltmarsh M. Polyphenols: antioxidants and beyond. Am J Clin Nutr. 2005; 81: 215S-217S.
6. Procházkováa D, Boušováb I, Wilhelmováa N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 2011; 82: 513-523.
7. Cowan MM. Plants products as antimicrobial agents. Clin Microbiol Rev. 1999; 12(4); 564-582.
8. Motamed SM, Naghibi F. Antioxydant activity of some edible plants of the Turkmen Sahara region in northern Iran. Food Chem. 2010; 119; 1637-1642.
9. Ljubuncic P, Song H, Cogan U, Azaizeh H, Bomzon A. The effects of aqueous extracts prepared from the leaves of Pistacia lentiscusin experimental liver disease. J Ethnopharmacol. 2005; 100: 198-204.
10. Singleton VL, Rossi JA. Colorimetry of total phenolics whith phosphomolibdic phosphotungstic acids reagents. Am J Enol Vitic. 1965; 16: 144-158.
11. Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006; 97: 654-660.
12. Ceylan O, Sahin MD, Avaz S. Antibacterial activity of Corylus colurna L. (Betulaceae) and Prunus divaricata ledep. subsp. divaricata (Rosaceae) from Usak, Turkey. Bulg J Agr Sci. 2013; 19(6): 1204-1207.
13. Benslama A, Harrar A, Gul F, Demirtas I. In vitro antioxidant, antibacterial activities and HPLC-TOF/MS analysis of Anvillea radiata (Asteraceae) extracts. Curr Nutr Food Sci. 2018; 14: 1-8.
14. Huang B, Ke HB, He JS, Ban, XQ, Zeng H, Wang YW. Extracts of Halenia elliptica exhibit antioxidant properties in vitro and in vivo. Food Chem Toxicol. 2011; 49; 185-190.
15. Benslama A, Harrar A, Gul F, Demirtas I. Phenolic compounds, antioxidant and antibacterial activities of Zizyphus lotus L. leaves extracts. Nat Prod J. 2017; 7(4): 316-322.
16. Bencheikh D, Gueddah A, Soualat K, Ben-aissi H, Benslama A, Harrar A. Polyphenolic contents, antioxidant and antibacterial activities of aqueous extracts of Eucalyptus globulus L. and Trigonella foenum-greacum L. J Appl Biol Sci. 2021; 15(1): 53-63.
17. Benslama A, Deghima A, Righi N. Assessment of Total Phenolic Content and Antioxidant Activity of Ficus carica and Olea europaea L. Leaves Extracts. Curr Nut Food Sci. 2018; 14: 1-5
18. Sasidharan Y, Chen Y, Saravanan D, Sundram KM, Yoga L. Extraction, isolation and characterization of bioactive compounds from plants' extracts. Afr J Tradit Complem Altern Med. 2011; 8(1): 1-10.
19. Daoudi A, Sabiri M, Bammou M, Zair T, Ibijbijen J, Nassiri L. Valorisation des extraits de trois espèces du genre Urtica: Urtica urens L., Urtica membranacea (Poiret) et Urtica pilulifera L. J Appl Biosci. 2015; 87: 8094-8104.
20. Keskes H, Mnafgui K, Hamden K, Damak M, ElFeki A, Allouche N. In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia. Asian Pac J Trop Biomed. 2014; 2: 649-655.
21. Assanga SB, Luján LM, Espinoza CL, Salido AAG, Angulo D, Rubio D. Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucid abuceras L. and Phoradendron californicum. BioMed Central Res Notes. 2015; 8: 2-14.
22. Lafka TI, Lazou A.E, Sinanoglou VJ, Lazos ES. Phenolic extracts from wild olive leaves and their potential as edible oils antioxidants. Foods. 2013; 2: 18-31.
23. Stanković MS. Total Phenolic Content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac J Sci. 2011; 33: 63-72.
24. Compean, KL, Ynalvez RA. Antimicrobial Activity of Plant Secondary Metabolites: A Review. Res J Med Plan. 2014; 8: 204-213.
25. Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol. 2012; 23: 174-181.
26. Craft BD, Kerrihard AL, Amarowicz R, Pegg, RB. Phenol-based antioxidants and the in vitro methods used for their assessment. Comp Rev Food Sci Food Saf. 2012; 1: 148-173.
27. Nur Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J. 2013; 21(2): 143-152.
28. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem. 2001; 49: 5165-5170.
29. Floegel A, Kim DO, Chung SJ, Sung I, Koo SI, Chun OK. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Comp Anal. 2011; 24: 1043-1048.
30. Hu JP, Calomme M, Lasure A. Structure-activity relationship of flavonoids with superoxide scavenging activity. Biol Trace Elem Res. 1995; 47: 327-331.
31. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power: the FRAP assay. Anal Bioch. 1996; 239: 70-76.
32. Benslama A, Harrar A. Free radicals scavenging activity and reducing power of two Algerian Sahara medicinal plants extracts. Int J Herb Med. 2016; 4(6); 158-161.
33. Pulido R, Bravo L, Saura-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem. 2000; 48: 3396-3402.
34. Chanda S, Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. Afr J Mic Res. 2009; 13: 981‐996.
35. Parejo I, Viladomat F, Bastida J, Rosas-Romero A, Saavedra, G, Murcia MA. Investigation of Bolivian plant extracts for their radical scavenging activity and antioxidant activity. Life Sci. 2003; 73: 1667-1681.
How to Cite
Benslama, A.; Daci, S.; Nabti, L.; Bendif, H.; Harrar, A. Assessment of Polyphenols Contents, Antibacterial and Antioxidant Activities of Origanum Majorana Extracts. European Journal of Biological Research 2021, 11, 509-518.
Research Articles