Physicochemical characterization and evaluation of the antioxidant activities of essential oil extracted from Eucalyptus globulus
Keywords:
Extraction, Eucalyptus globulus, Antioxidant, Essential oil, Oxidative stress
Abstract
This work was conducted as part of evaluation of the antioxidant activities of the essential oil extracted from a plant that belongs to the family of Myrtaceae: Eucalyptus globulus. The extraction of the essential oil was carried out by hydrodistillation and followed by extraction yield determination and physicochemical analysis. Then, the evaluation of the antioxidant activity was performed according to the method of DPPH free radical scavenging and the determination of total antioxidant capacity. The extraction of the essential oil gave a content of 0.41 ± 0.01%. The analytical study of the physicochemical properties of the essential oil of E. globulus showed that this plant presented an essential oil of acceptable quality and in conformity with the standard. The results of the evaluation of the antioxidant activity showed that this essential oil has interesting antiradical properties. It was manifested by a low value of IC50 (0.017 mg /ml) compared to the standard antioxidant (ascorbic acid). It was noticed that the essential oil of E. globulus has an antioxidant capacity of the order of 19 ± 0.01 mg AAE/g. This result showed that the essential oil of E. globulus has a powerful antioxidant power by reducing phosphomolybdate. Thus, the essential oil of E. globulus appeared effective in reducing oxidative reactions.
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References
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37. Pietta PG. Flavonoids as Antioxidants. J Nat Prod. 2000; 63(7): 1035-1042.
38. Hasegawa T, Takano F, Takata T, Niiyama M, Ohta T. Bioactive monoterpene glycosides conjugated with gallic acid from the leaves of Eucalyptus globulus. Phytochemistry. 2008; 69(3): 747-53.
39. Vázquez G, Santos J, Sonia Freire M, Antorrena G, González-Álvarez J. Extraction of antioxidants from eucalyptus (Eucalyptus globulus) bark. Wood Sci Technol. 2011; 46: 443-457.
40. Akolade JO, Olajide OO, Afolayan MO, Akande SA, Idowu DI, Orishadipe AT. Chemical composition, antioxidant and cytotoxic effects of Eucalyptus globulus grown in north-central Nigeria. J Nat Prod Plant Res. 2012; 2(1): 1-8.
41. Martins MDR, Arantes S, Candeias F, Tinoco MT, Cruz-Morais J. Antioxidant, antimicrobial and toxicological properties of Schinus molle L. essential oils. J Ethnopharmacol. 2014; 151: 485-492.
42. Vecchio MG, Loganes C, Minto C. Beneficial and Healthy Properties of Eucalyptus Plants: A Great Potential Use. Open Agric J. 2016; 10(1): 52-57.
43. Harkat-Madouri L, Boudria A, Khodir M, Bey-Ould Si Said Z, Peggy R, Grenier D, et al. Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalyptus globulus from Algeria. Ind Crop Prod. 2015; 78: 148-153.
44. Leela N K, Archana G, Anandaraj M. Chemoprofiling of clove fruit oil and its antioxidant potential. ICAR – Indian Institute of Spices Research, Marikunnu P.O., Kozhikode- 673012, Kerala. 2013.
45. Sharififar F, Dehghn-Nudeh G, Mirtajaldini M. Major flavonoids with antioxidant activity from Teucrium polium L. Food Chem. 2009; 112: 885-888.
46. Khan RA, Khan MR, Sahreen S. Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens. Chem Central J. 2012; 6(1): 43.
47. Steenkamp V, Nkwane O, Tonder J, Dinsmore A, Gulumian M. Evaluation of the phenolic and flavonoid contents and radical scavenging activity of three Southern African medicinal plants. Afr J Pharm Pharmacol. 2013; 7(13): 703-709.
2. WHO traditional medicine strategy for 2014-2023. WHO 2013.
3. Ladoh Yemeda CF, Dibong SD, Nyegue MA, Djembissi Talla RP, Lenta Ndjakou B, Mpondo Mpondo E, et al. Antioxidant activity of methanolic extracts of Phragmanthera capitata (Loranthaceae) harvested from Citrus sinensis. J. Appl Biosci. 2014; 84: 7636-7643.
4. Buronzo A. Anti-aging: do you choose the “good” antioxidants? Plant and Well-being. 2016; no. 26.
5. Bidie P, Banga B, Guessan N, Adou F. Antioxidant activities of ten medicinal plants from the Ivorian pharmacopoeia. Sci Nature. 2011; 8(1): 1-11.
6. Abedini A. Lamiaceae, selected by screening extracts of 42 plants, doctoral thesis in drug science, University of Lille, France; 2013: P12.
7. Zenasni L. Study of chemical polymorphism of essential oils of Thymus satureioides Coss and Origanum compactum Benth and of the genus Nepeta and evaluation of their antibacterial property. Doctoral thesis. Mohammed V University, Rabat; 2014; 155 p.
8. Girodon M. Metabolic Engineering of Plant Secondary Metabolism. Ed. Kluwer Academic, Dordrecht. Netherlands. 2010; 286 p.
9. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: how are they linked? Free Radic. Biol. Med. 2010; 49: 1603-1616.
10. Buronso AM. Great guide to essential oils, health, beauty and well-being. Hachette Pratique; 2008.
11. Kheyer N, Dahia M, Belhamer K. Study of the antibacterial activity of essential oils of Inula viscosa, Salvia officinalis and Laurus nobilis from the region of Bejaia. Algerian J Nat Prod. 2014; 2: 18-26.
12. AFNOR. Collection of standards: essential oils. Volume 2. Monographs relating to essential oils. AFNOR, Paris. 2000: 661-663.
13. Sanchez-Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int. 2002; 8: 121-137.
14. Torres R, Faini F, Modak B, Urbina F, Labbe´ C, Guerrero J. Antioxidant activity of coumarins and flavonols from the resinous exudate of Haplopappus multifolius. Phytochemistry. 2006; 67: 984-987.
15. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999; 269(2): 337-341.
16. Taleb-Toudert K. Extraction and characterization of essential oils from ten aromatic plants from the Kabyle region (North of Algeria). Evaluation of their effects on the cowpea weevil Callosobruchus maculatus (Coleoptera: Bruchidae); 2015; 39.
17. Pereira S, Freire SRC, Neto P, Silvestre JD, Silva MSA. Chemical composition of the essential oil distilled from the fruits of Eucalyptus globulus grown in Portugal. Flavour Fragr J. 2005; 20: 407-409.
18. Mulyaningsih S, Sporer F, Zimmermann S, Reichling J, Wink M. Synergistic properties of the terpenoids aromadendrene and 1,8-cineole from the essential oil of Eucalyptus globulus against antibiotic susceptible and antibiotic resistant pathogens. Phytomed. 2010; 17: 1061-1066.
19. Aouidet S. Study of the impact of Eucalyptus globulus essential oils on Rhyzopertha dominica: Toxicol Biomarker Aspect; 2016; 25.
20. Evans WC. Trease and Evan's Pharmacognosy. Sanders, 14th edn; 1998: 48- 65.
21. Makhlouf H. Rosemary and clove essential oils: analytical approach and antioxidant activity on edible oil. Engineer thesis, I.N.A., Algiers. 2002; 82.
22. Emara S, Shalaby AE. Seasonal variation of fixed and volatile oil percentage of four Eucalyptus spp. related to lamina anatomy. Afr J Plant Sci. 2011; 5(6): 353-359.
23. Bruneton J. Pharmacognosy, phytochemistry of medicinal plants. Editing. Technical and documentary, 3rd edn. 1993; 634.
24. Bennadja S, Tlili Ait Kaki Y, Djahoudi A, Hadef Y, Chefrour A. Antibiotic activity of the essential oil of laurel (Laurus nobilis L.) on eight bacterial strains. J Life Sci. 2013; 7(8): 814-819.
25. Bagheri H, Abdul Manap MYB, Solati Z. Antioxidant activity of Piper nigrum L. essential oil extracted by supercritical CO2 extraction and hydro-distillation. Talanta. 2014; 121: 220-228.
26. Rabiai M. Physicochemical study and evaluation of the biological activity of an essential oil and the aqueous extract of Eucalyptus globulus from the Msila region. Master's thesis, University of Msila. 2014.
27. Mendes Silva S, Yae Abe S, Seigi Murakami F, Frensch G, Marques Francisco A, Nakashima T. Essential Oils from Different Plant Parts of Eucalyptus cinerea F. Muell. ex Benth. (Myrtaceae) as a Source of 1,8-Cineole and Their Bioactivities. Pharmaceuticals. 2011; 4: 1535-1550.
28. Ben Hassine D, Abderrabba M, Yvon Y, Lebrihi A, Mathieu F, Couderc F, et al. Chemical Composition and in Vitro Evaluation of the Antioxidant and Antimicrobial Activities of Eucalyptus gillii Essential Oil and Extracts. Molecules. 2012; 17(8): 9540-9558.
29. Hussain AI, Anwar F, Sherazi STH, Przybylski R. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem. 2008; 108: 986-995.
30. Bourachouche K, Boudei A. Physicochemical characterization of edible vegetable oils, University of Bejaia; 2017; 42.
31. De Cliff S, Harerimana PC. Extraction of the complete essential oil from the flowers of Cananga odorata in the Imbo plain: towards the popularization of a new sector of industrial plants in Burundi. J Univ Burundi. 2013; 28: 1-17.
32. Hilan C, Sfeir R, Jawish D, Aitour S. Essential oils of certain Lebanese medicinal plants of the Lamiaceae family. Lebanese Sci J. 2006; 7(2): 13-22.
33. Mishra Arun K, Neelum S, Amrita M, Ashoke KG, Shivesh J, Pronobesh C. Phytochemical Screening and Antioxidant Activity of essential oil of Eucalyptus leaf. Pharmacogn J. 2010; 2(16): 25.
34. Aidi Wannes W, Mhamdi B, Sriti J, Ben Jemia M, Ouchikh O, Hamdaoui Marzouk B. Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf stem and flower. Food Chem Toxicol. 2010; 48: 1362-1370.
35. Apostolou A, Stagos D, Galitsiou E, Spyrou A, Haroutounian S, Portesis N. Assessment of polyphenolic content, antioxidant activity, protection against ROS-induced DNA damage and anticancer activity of Vitis vinifera stem extracts. Food Chem Toxicol. 2013; 61: 60-68.
36. Bey-Ould Si Said Z, Haddadi-Guemghara H, Boulekbache-Makhlouf L, Rigoub P, Remini H, Adjaouda A, et al. Essential oils composition, antibacterial and antioxidant activities of hydrodistillated extract of Eucalyptus globulus fruits. Ind Crop Prod. 2016; 89: 167-175.
37. Pietta PG. Flavonoids as Antioxidants. J Nat Prod. 2000; 63(7): 1035-1042.
38. Hasegawa T, Takano F, Takata T, Niiyama M, Ohta T. Bioactive monoterpene glycosides conjugated with gallic acid from the leaves of Eucalyptus globulus. Phytochemistry. 2008; 69(3): 747-53.
39. Vázquez G, Santos J, Sonia Freire M, Antorrena G, González-Álvarez J. Extraction of antioxidants from eucalyptus (Eucalyptus globulus) bark. Wood Sci Technol. 2011; 46: 443-457.
40. Akolade JO, Olajide OO, Afolayan MO, Akande SA, Idowu DI, Orishadipe AT. Chemical composition, antioxidant and cytotoxic effects of Eucalyptus globulus grown in north-central Nigeria. J Nat Prod Plant Res. 2012; 2(1): 1-8.
41. Martins MDR, Arantes S, Candeias F, Tinoco MT, Cruz-Morais J. Antioxidant, antimicrobial and toxicological properties of Schinus molle L. essential oils. J Ethnopharmacol. 2014; 151: 485-492.
42. Vecchio MG, Loganes C, Minto C. Beneficial and Healthy Properties of Eucalyptus Plants: A Great Potential Use. Open Agric J. 2016; 10(1): 52-57.
43. Harkat-Madouri L, Boudria A, Khodir M, Bey-Ould Si Said Z, Peggy R, Grenier D, et al. Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalyptus globulus from Algeria. Ind Crop Prod. 2015; 78: 148-153.
44. Leela N K, Archana G, Anandaraj M. Chemoprofiling of clove fruit oil and its antioxidant potential. ICAR – Indian Institute of Spices Research, Marikunnu P.O., Kozhikode- 673012, Kerala. 2013.
45. Sharififar F, Dehghn-Nudeh G, Mirtajaldini M. Major flavonoids with antioxidant activity from Teucrium polium L. Food Chem. 2009; 112: 885-888.
46. Khan RA, Khan MR, Sahreen S. Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens. Chem Central J. 2012; 6(1): 43.
47. Steenkamp V, Nkwane O, Tonder J, Dinsmore A, Gulumian M. Evaluation of the phenolic and flavonoid contents and radical scavenging activity of three Southern African medicinal plants. Afr J Pharm Pharmacol. 2013; 7(13): 703-709.
Published
2021-06-25
How to Cite
(1)
Belkhodja, H.; Bouhadi, D.; Medjadel, B.; Brakna, A. Physicochemical Characterization and Evaluation of the Antioxidant Activities of Essential Oil Extracted from Eucalyptus Globulus. European Journal of Biological Research 2021, 11, 315-324.
Section
Research Articles
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