MicroMedicine http://journals.tmkarpinski.com/index.php/mmed <p><strong>ISSN 2449-8947</strong><br><strong><a title="MNiSW points: 8" href="http://www.nauka.gov.pl/g2/oryginal/2016_12/c5c2fcb0c283a9eb3d1081020fd3178c.pdf" target="_blank" rel="noopener">MNiSW points 2016: 8</a></strong><br><strong><a title="ICV: 86.09" href="http://journals.indexcopernicus.com/MicroMedicine,p24783445,3.html" target="_blank" rel="noopener">ICV 2017: 86.09</a></strong></p> en-US mmed@tmkarpinski.lh.pl (Joanna Bródka) mmed@tmkarpinski.lh.pl (Joanna Bródka) Sat, 22 Jun 2019 19:32:02 +0200 OJS http://blogs.law.harvard.edu/tech/rss 60 Antibacterial activity of methanol extract of Lawsonia inermis against uropathogenic bacteria http://journals.tmkarpinski.com/index.php/mmed/article/view/233 <p>Urinary tract infections one of the most common bacterial diseases caused by microbes such as bacteria overcoming the body's defenses in the urinary tract. Extensive studies are needed to identify the bacteria of the infectious agent and to determine the pattern of drug resistance and the identification of effective drugs for proper treatment. Therefore, the purpose of this study was to determine the antibiotic resistance pattern of urinary pathogens and compare it with methanol extract of <em>Lawsonia inermis</em>. Urine samples were collected using the mid-stream "clean catch" technique and examined bacteriologically using standard procedures. The antibiotic resistance pattern of each uropathogen isolated was carried by Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standards Institute guidelines. Methanol extract of the plant was prepared by maceration method and its inhibitory effect on uropathogen isolates studied using well diffusion assay. Based on obtained results, the most common uropathogen isolated was <em>Escherichia coli</em> and the most frequent uropathogens were Gram negative rod bacteria. Most resistant antibiotics were Cefazolin, Ampicillin, Vancomycin and Nitrofurantoin. Methanol extract of <em>Lawsonia inermis</em> showed good antibacterial potential against all uropathogen bacterial isolates. The results suggest that the <em>Lawsonia inermis</em> possess antibacterial properties that support the folk medicinal use of this plant.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.3473381">http://dx.doi.org/10.5281/zenodo.3473381</a></strong></p> Salim Shahabinejad, Ashraf Kariminik ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 http://journals.tmkarpinski.com/index.php/mmed/article/view/233 Sat, 22 Jun 2019 00:00:00 +0200 Extended spectrum and metallo-beta-lactamase Pseudomonas species from poultry and piggery waste http://journals.tmkarpinski.com/index.php/mmed/article/view/236 <p>Beta-lactamase producing bacteria have become a public health burden due to antibiotics usage in livestock production. This study was carried out to detect extended spectrum beta-lactamase (ESBL) and metallo-beta-lactamase (MBL) producing <em>Pseudomonas</em> spp. from poultry droppings and piggery dung in Ibadan. Poultry droppings and piggery dung were collected from the University of Ibadan livestock farms while isolation of <em>Pseudomonas</em> spp. was done using <em>Pseudomonas</em> base agar supplemented with <em>Pseudomonas</em> C-N supplement and were conventionally characterized. Detection of ESBL and MBL producing isolates were by double disc synergy test and imipenem-EDTA combined disc test respectively. Antimicrobial susceptibility test was by disc diffusion method against trimethoprim (5 µg), amoxicillin/clavulanate (30 µg), cefotaxime (30 µg), ceftazidime (30 µg), cefepime (30 µg), aztreonam (30 µg), imipenem (10 µg), gentamicin (10 µg) and ciprofloxacin (10 µg). A total of 108 <em>Pseudomonas</em> spp. were isolated comprising 53.7% from poultry droppings and 46.3% from piggery dung. The isolates include <em>P. aeruginosa</em> (63.0%), <em>P. putida</em> (24.0%) and <em>P. stutzeri</em> (13.0%). While the ESBL producers were <em>P. aeruginosa</em> (10.2%) and <em>P. stutzeri</em> (1.9%), none of the isolates produced MBL. However, 63.6% the ESBL producers showed resistance to trimethoprim while 61.5% were multidrug resistant. The high prevalence of antibiotics resistance and multidrug resistant strains observed among the <em>Pseudomonas</em> spp. infer that poultry droppings and piggery dung can serves as a reservoir for growth and dissemination of clinically significant antibiotics resistance among bacterial species.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.3489572">http://dx.doi.org/10.5281/zenodo.3489572</a></strong></p> Olutayo Israel Falodun, Emmanuel Olugbenga Ikusika ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 http://journals.tmkarpinski.com/index.php/mmed/article/view/236 Mon, 14 Oct 2019 00:00:00 +0200 Mesiodens - case report http://journals.tmkarpinski.com/index.php/mmed/article/view/146 <p>Mesiodens is supernumerary tooth which is situated between the central incisors. The most frequent type of supernumerary tooth is a mesiodens. Supernumerary teeth may also disturb the teeth eruption and cause several complications, such as ectopic eruption and midline diastema. A 13 year-old girl (VA), with mesiodens in the upper jaw was presented in this article. She has esthetics, diastema mediana and ectopic position of the left central incisor was present. Orthopantomogram investigations revealed the presences of excessive teeth, in the upper jaw. According to the orthopantomogram’s and clinical findings, the patient was sent at the Department of Oral Surgery for the extraction of the mesiodens. Early and regular dental visits will help to diagnose and avoid problems that mesiodens can cause. Extraction of the mesiodens in the early mixed dentition was recommended, to decrease the risk of clinical complications, lighter spontaneous eruption of adjacent teeth and reduced need for orthodontic treatment after surgery.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.3252560">http://dx.doi.org/10.5281/zenodo.3252560</a></strong></p> Vesna Ambarkova, Lejla Kadrija, Naziktere Ramadani ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 http://journals.tmkarpinski.com/index.php/mmed/article/view/146 Sat, 22 Jun 2019 19:35:39 +0200