MicroMedicine http://journals.tmkarpinski.com/index.php/mmed <p><strong>ISSN 2449-8947</strong><br><strong><a title="MNiSW points: 8" href="http://www.nauka.gov.pl/g2/oryginal/2016_12/c5c2fcb0c283a9eb3d1081020fd3178c.pdf" target="_blank" rel="noopener">MNiSW points 2016: 8</a></strong><br><a title="ICV: 86.27" href="http://journals.indexcopernicus.com/MicroMedicine,p24783445,3.html" target="_blank" rel="noopener"><strong>ICV 2015: 86.27</strong></a></p> TMKarpinski Publisher en-US MicroMedicine 2449-8947 Chromatographic and antiproliferative assessment of the aerial root of Ficus thonningii Blume (Moraceae) http://journals.tmkarpinski.com/index.php/mmed/article/view/9 <div> <p><em>Ficus thonningii&nbsp;</em>(Blume) has long history of use for variety of ailments. The hot aqueous extract of&nbsp;<em>Ficus thonningii</em>&nbsp;aerial root (FT) was obtained by infusion. The antiproliferative activity of FT was evaluated using&nbsp;<em>Sorghum bicolor&nbsp;</em>seed radicle over a period of 24 h to 96 h. The mean radicle length (mm), percentage inhibition and percentage growth were calculated. Chemical characterization of FT was done using chromatographic techniques. Thin layer chromatography revealed the presence of β-sitosterol. High performance liquid chromatography showed ten peaks with gallic acid, tannins, caffeic acid, rutin, ferulic acid and morin eluting at 3.530, 3.928, 4.668, 6.706, 7.669 and 18.844 minutes respectively. Compared with negative control, FT at 1 mg/ml to 32 mg/ml significantly (p&lt;0.0001) inhibited&nbsp;<em>S</em>.&nbsp;<em>bicolor&nbsp;</em>seed radicle growth over 24 h-96 h. At 96h, FT dose-dependently inhibited&nbsp;<em>S. bicolor</em>&nbsp;seed growth, giving a percentage inhibition of 20.31%, 24.30%, 31.71%, 53.23%, 78.74%, 95.37% at 1 mg/ml, 2 mg/ml, 4 mg/ml, 8 mg/ml, 16 mg/ml, 32 mg/ml, respectively. Methotrexate 50 µg/ml used as the positive control gave inhibition of 70.62% at 96h. The result revealed the potential of FT to inhibit rapid proliferating cells of&nbsp;<em>S. bicolor</em>&nbsp;seed radicle and by extension cancer cells.</p> <p><strong>DOI:</strong> <a href="http://dx.doi.org/10.5281/zenodo.1143651">http://dx.doi.org/10.5281/zenodo.1143651</a></p> </div> Maryam Odufa Adamu Solomon Fidelis Ameh Utibe Abasi Okon Ettah Samuel Ehiabhi Okhale ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-06-30 2018-06-30 6 1 1 9 Predictors of residual disease after loop electrosurgical excision procedure http://journals.tmkarpinski.com/index.php/mmed/article/view/10 <div> <p>The study aims to evaluate the importance of resection margins in the risk of residual disease (RD) and to investigate other factors that could potentially predict RD before patients engage in follow-up. Eighty-six women with a histologically confirmed diagnosis of cervical intraepithelial neoplasms (CIN) treated by loop electrosurgical excision procedure (LEEP), were included in this retrospective study, between January 2015 and May 2016. Age, smoking habit, menopause status, and LEEP margins were evaluated as possible predictors of RD. The mean age at diagnosis was 35.8 years (range 18-61). The mean follow-up period was 12 months. 11.6% of patients (09/86) were lost in follow-up. 64% of patients (55/86) had clear margins in the specimen and 34.8% of patients had positive surgical margins (30/86). In 1.2% of patients (01/86) the resection margins were uncertain. RD was demonstrated by positive Pap Smear and by colposcopy-guided biopsy in 26.7% of patients (23/86). We found significant differences in the frequency of RD depending on the status of margins: 65.2% of cases with positive margins vs. 24.5% of cases with negative margins (p&lt;0.0001). Multivariate analysis showed that only high-grade squamous intraepithelial lesion (H-SIL) detection in cervical biopsy and status of the LEEP margins were significantly predictive of RD (OR 5.4, 95%CI 1.08-27.7, p&lt;0.05 and OR 7.05, 95%CI 2.1-23.1, p=0.001; respectively). The combination of histological examination of resection margins plus H-SIL detection in cervical biopsy would help to classify LEEP-treated patients into categories of different risk levels of residual disease.</p> <p><strong>DOI:</strong> <a href="http://dx.doi.org/10.5281/zenodo.1173700">http://dx.doi.org/10.5281/zenodo.1173700</a></p> </div> Ana Carolina Dantas Fabricio de Hollanda Ana Katherine Gonçalves Carlos André Jatobá Teresa Neumann Dantas Fabricio Adalberto Fabricio da Silva Ricardo Ney Cobucci ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-06-30 2018-06-30 6 1 10 15 Exposure to stress minimizes the zone of antimicrobial action: a phenotypic demonstration with six Acinetobacter baumannii strains http://journals.tmkarpinski.com/index.php/mmed/article/view/11 <p><strong>Aim:</strong> To phenotypically study the role of domestic environmental stress in the emergence of antimicrobial resistance in <em>Acinetobacter baumannii</em>. <strong>Materials and Methods: </strong>Six strains of <em>A. baumannii</em> were initially subjected to AST and then were exposed to various stresses (temperature, pH and random combinations). Stressed cells were subcultured and then subjected for AST. The ZOIs before and after exposure to stress were compared. Statistical analysis was done using Student t-test at p &lt; 0.10. <strong>Results:</strong> Exposure to stresses and combination of stresses resulted in substantial reduction in the ZOIs. Stress hardening was associated with further reduction in ZOIs. <strong>Conclusion:</strong> Exposure to domestic environmental stress imparted a significant and substantial reduction in the susceptibility of <em>A. baumannii</em> strains to antibiotics.</p> <p><strong>DOI:</strong> <a href="http://dx.doi.org/10.5281/zenodo.1184152">http://dx.doi.org/10.5281/zenodo.1184152</a></p> A. Ebinesh G. S. Vijaykumar T. S. Kiran ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-06-30 2018-06-30 6 1 16 35 Orthodontic anomalies in mixed dentition http://journals.tmkarpinski.com/index.php/mmed/article/view/25 <p><strong>Aim:</strong> To estimate the frequency of orthodontic anomalies in the period of mixed dentition in children, and to highlight the importance of prompt treatment in order to prevent more serious disorders of the child oral health.</p> <p><strong>Materials and methods:</strong> For accomplishing the goal we’ve conducted systematic and control check-ups on children at the age of 9; 95 children from urban areas, and 68 from rural areas.</p> <p><strong>Results:</strong> Of 95 analyzed children from urban areas with ethnic Macedonian population, 81 have an orthodontic anomaly. Nearly half of them i.e. 39 have mobile appliances. The most common anomaly are protrusion of teeth standing at 28.3%, a deep bite with 21% and crowding with 17.2%. Of 68 analyzed children from rural areas of ethnic Albanians, 54 have an orthodontic anomaly, while only 3 children wear mobile appliances. The most common anomalies are both crowding and maxillary protrusion of teeth with an equal 27.7%. If we compare the results: the occurrence of orthodontic anomalies is slightly higher in the Macedonian population.</p> <p><strong>Conclusion:</strong> Orthodontic anomalies are diagnosed during regular systematic dental check-ups for children aged 7-13. The period of mixed dentition, which is characterized by an intense growth of the jaws, is ideal for orthodontic treatment. The parents have opportunities to inform themselves of the orthodontic anomaly of their children and promptly visit an orthodontist. With properly conducted activities on behalf of the preventive teams, we can severely decrease the percentage of children with orthodontic irregularities.</p> <p><strong>DOI:</strong> <a href="http://dx.doi.org/10.5281/zenodo.1227309">http://dx.doi.org/10.5281/zenodo.1227309</a></p> Gordana Todorovska Vesna Ambarkova Olga Kokocheva–Ivanovska Biljana Dzipunova Natasha Tosheska-Spasova Katerina Sibinoska ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-04-23 2018-04-23 6 1 36 46 Effect of age on chemical element contents in female thyroid investigated by some nuclear analytical methods http://journals.tmkarpinski.com/index.php/mmed/article/view/33 <p>A prevalence of thyroid dysfunction is higher in the elderly as compared to the younger population. An excess or deficiency of chemical element contents in thyroid may play an important role in goitro- and carcinogenesis of gland. The variation with age of the mass fraction of twenty chemical elements (Ag, Br, Ca, Cl, Co, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Rb, Sb, Sc, Se, Sr, and Zn) in intact (normal) thyroid of 33 females (mean age 54.5 years, range 3.5-87) was investigated by energy dispersive X-ray fluorescent analysis and instrumental neutron activation analysis with high resolution spectrometry of short- and long-lived radionuclides. This work revealed that there is an increase in Br, Ca, Co, Fe, Rb, Sb, Se, and Zn mass fraction, as well as a decrease in Cl mass fraction in the normal thyroid of female during a lifespan. Therefore, a goitrogenic and carcinogenic effect of inadequate Br, Ca, Co, Fe, Rb, Sb, Se, and Zn level in the thyroid of old females with increasing age may be assumed.</p> Vladimir Zaichick Sofia Zaichick ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-03-20 2018-03-20 6 1 47 61