RNA isolation from paraffinated material of histological´s prepared slides

Matheus Moreira Perez, Alexandre Luiz Affonso Fonseca, Fábio Prosdocimi, Bianca Bianco, Fernando Luiz Affonso Fonseca, Beatriz da Costa Aguiar Alves

Abstract


The parafinization of solid biopsies allows a better preservation and preparation of microscopy slides used for histological evaluations. Techniques of RNA extraction are suitable to be applied in microtomized paraffinated material, but the extraction of paraffinic material already disposed in a slide has not yet been described and can be useful for gene expression studies. We adapted the RNA isolation technique of paraffinated material for slide-laid paraffinited material, using the Rneasy® FFPE Kit. Total RNA so isolated is suitable to gene expression analysis by RT-qPCR.


Keywords


Paraffinated material; Slides; RNA isolation

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References


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