Comparison of protein isolation methods from clear cell Renal Cell Carcinoma tissue

Konrad Kamil Hus, Krzysztof Ossoliński, Marcin Jaromin, Tadeusz Ossoliński, Anna Ossolińska, Zygmunt Dobrowolski, Grażyna Groszek, Aleksandra Bocian, Andrzej Łyskowski

Abstract


This study presents the comparison of three protein extraction methods in the investigations of clear cell Renal Cell Carcinoma tissue. For protein isolation, we applied: phenol extraction according to Hurkman and Tanaka (1986) protocol (method 1), whole tissue lysis in urea-containing buffer (method 2) and commercially available protein isolation kit (2-D Clean-up Kit) (method 3). Statistical analysis indicated that the additional preparation steps including extraction and purification of proteins by 2-D Clean-up Kit significantly increased the quality of obtained data. We believe that gathered information could be a valuable lead for researchers involved in proteomic studies of renal tissue.


Keywords


Biomarkers; Clear cell Renal Cell Carcinoma; Protein isolation; Proteomics; Renal tissue

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References


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