Partial purification and antidiabetic effect of bioactive compounds isolated from medicinal plants

Twinkle S. Bansode, Amit Gupta, Bharat Shinde, B. K. Salalkar


Antidiabetic studies were conducted in human whole blood using different medicinal plant products (Trigonella foenum graecum, seeds; Syzygium cumini, seeds; Salavadora persica, leaves and Terminalia chebula, seeds). The objective of our study is to screen fractions of these four different medicinal plant products on diabetic human whole blood samples. For these studies, our group evaluated secondary metabolites through thin layer chromatography (TLC) and also determined its activity on diabetic human whole blood samples in order to determine total cellular content, free hemoglobin in the supernatant and also estimated its glucose content. The results of these studies claimed that all these fractions isolated from these medicinal plant productsshowed antidiabetic effect at lower doses because of decline in total cellular content, free hemoglobin in the supernatant and glucose content but maximum effect observed in case of fraction isolated from Syzygium cumini and Salavadora persica. Overall, this study claimed that all these plant fractions showed anti-diabetic activity.


Trigonella foenum graecum; Syzygium cumini; Salavadora persica; Terminalia chebula; Antidiabetic

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