Antibacterial activity of methanol extract of Lawsonia inermis against uropathogenic bacteria

  • Salim Shahabinejad Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
  • Ashraf Kariminik Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
Keywords: Urinary tract infection, Antibacterial activity, Antibiotic, Lawsonia inermis

Abstract

Urinary tract infections one of the most common bacterial diseases caused by microbes such as bacteria overcoming the body's defenses in the urinary tract. Extensive studies are needed to identify the bacteria of the infectious agent and to determine the pattern of drug resistance and the identification of effective drugs for proper treatment. Therefore, the purpose of this study was to determine the antibiotic resistance pattern of urinary pathogens and compare it with methanol extract of Lawsonia inermis. Urine samples were collected using the mid-stream "clean catch" technique and examined bacteriologically using standard procedures. The antibiotic resistance pattern of each uropathogen isolated was carried by Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standards Institute guidelines. Methanol extract of the plant was prepared by maceration method and its inhibitory effect on uropathogen isolates studied using well diffusion assay. Based on obtained results, the most common uropathogen isolated was Escherichia coli and the most frequent uropathogens were Gram negative rod bacteria. Most resistant antibiotics were Cefazolin, Ampicillin, Vancomycin and Nitrofurantoin. Methanol extract of Lawsonia inermis showed good antibacterial potential against all uropathogen bacterial isolates. The results suggest that the Lawsonia inermis possess antibacterial properties that support the folk medicinal use of this plant.

DOI: http://dx.doi.org/10.5281/zenodo.3473381

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Published
2019-06-22
How to Cite
Shahabinejad, S., & Kariminik, A. (2019). Antibacterial activity of methanol extract of Lawsonia inermis against uropathogenic bacteria. MicroMedicine, 7(2), 31-36. Retrieved from http://journals.tmkarpinski.com/index.php/mmed/article/view/233
Section
Research Articles

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