Bioconcentration of hexavalent chromium in Cirrhinus mrigala (Ham 1822): effect on haematological parameters

B. Mallesh, P. K. Pandey, Kundan Kumar, A. Vennila, Saurav Kumar

Abstract


The study was carried out with intents to measure the relative bioconcentration of chromium (Cr6+) in different vital organs, and to assess the effect of chromium on hematology in Cirrhinus mrigala. The fingerlings of C. mrigala were exposed to different concentrations of Cr6+ (LC50 for 96 h was estimated as 52.187 mg L-1) in the form of K2Cr2O7. For sub-lethal toxicity test, the fish were exposed to different concentrations of T0 (without chromium), T1 (3.479 mg L-1 Cr6+), T2: 5.218 mg L-1 Cr6+), and T3 (10.437 mg L-1 Cr6+) for 60 days. For estimation of bioaccumulation, the various vital organs (muscle, gills and liver) and haematological parameters, peripheral blood was collected. The chromium-exposed fish showed significantly (p<0.05) high chromium concentration in liver (61.91±0.73 µg g-1) followed by gills (16.67±0.08 µg g-1) and muscle (8.97±0.06 µg g-1). The effects of chromium on various haematolo- gical parameters showed a significant (p>0.05) decreased in the red blood cells (RBC), haemoglobin (Hb) content and packed cell volume (PCV) at the end of 60 days when compared to the control, whereas the white blood cells (WBC) and mean corpuscular volume (MCV) significantly (p<0.05) increased in the Cr6+ exposed groups. The abnormalities measured during this study are principally imperative because they are associated with bioaccumulation in vital tissues, impaired haematology at sub-lethal concentrations of Cr6+ which provides the early diagnostic tools to detect the toxicity of chromium pollution in aquatic environment.


Keywords


Chromium, Cirrhinus mrigala, haematology, bioconcentrationChromium; Cirrhinus mrigala; Haematology; Bioconcentration

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References


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