Antibacterial activity of rosemary, caraway and fennel essential oils



Introduction: Recently, interest in essential oils used in natural medicine, has been increasing. Essential oils are still being tested for their potential uses as an alternative remedies for the treatment of many infectious diseases. Objective: The aim of the study was to evaluate antibacterial properties of commercial essential oils (rosemary, caraway and fennel) to reduce the number of Staphylococcus aureus and Escherichia coli. Methods: The antibacterial activity of essential oils was investigated by agar dilution method. Results: The result of experiments showed that essential oils contained in microbiological media significantly reduced the number of S. aureus and E. coli cells. The best antibacterial properties possessed caraway oil: 1 mg/g for S. aureus and 10 mg/g for E. coli, weaker rosemary (5 mg/g) and fennel (20 mg/g) oils. Conclusion: Results confirmed the inhibiting effect of commercial essential oils on S. aureus and E. coli and provide a scientific ground for future research.


Escherichia coli, Staphylococcus aureus, rosemary, caraway, fennel

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Prabuseenivasan S, Jayakumar M, Ignacimuthu S. In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med 2006; 6:1-8.

Mahmoodi A, Roomiani L, Soltani M, Basti AA, Kamali S, Taheri S. Chemical composition and antibacterial activity of essential oils and extracts from Rosmarinus officinalis, Zataria multiflora, Anethum graveolens and Eucalyptus globulus. Global Vet 2012; 9:73-9.

Begum J, Bhuiyan MNI, Chowdhury JU, Hoque MN, Anwar MN. Antimicrobial activity of essential oil from seeds of Carum carvi and its composition. Bangladesh J Microbiol 2008; 25:85-9.

Diao W, Hu Q, Zhang H, Xu J. Chemical composition, antibacterial activity and mechanism of action of essential oil from seeds of fennel (Foeniculum vulgare Mill.). Food Contr 2014; 35:109-16.

Jalali-Heravi M, Moazeni RS, Sereshti H. Analysis of Iranian rosemary essential oil: Application of gas

chromatography–mass spectrometry combined with chemometrics. J Chromatogr A 2011; 1218:2569-76.

Minaiyan M, Ghannadi AR, Afsharipour M, Mahzouni P. Effects of extract and essential oil of Rosmarinus officinalis L. on TNBS-induced colitis in rats. Res Pharm Sci, 2011; 6:13-21.

Thippeswamy NB, Naidu KA, Achur RN. Antioxidant and antibacterial properties of phenolic extract from Carum carvi L. J Pharm Res 2013; 7:352-7.

Rahimi R, Ardekani MRS. Medicinal properties of Foeniculum vulgare Mill. in traditional Iranian medicine and modern phytotherapy. Chin J Integr Med, 2013; 19:73-9.

Bachir RG, Benali M. Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus. Asian Pac J Trop Biomed 2012; 2:739-42.

Fournier B, Philpott DJ. Recognition of Staphylococcus aureus by the innate immune system. Clin Microbiol Rev 2005; 3:521-40.

Jafari A, Aslani MM, Bouzari S. Escherichia coli: a brief review of diarrheagenic pathotypes and their role in diarrheal diseases in Iran. Iran J Microbiol 2012; 4:102-17.

Owlia P, Saderi H, Rasooli I, Sefdikon F. Antimicrobial characteristics of some herbal oils on Pseudomonas aeruginosa with special reference to their chemical compositions. Indian J Pharmacol Res, 2009; 8:107-14.

Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. J App Microbiol 1999; 86:985-90.

Aćimović MG, Oljača SI, Tešević VV, Todosijevića MM, Djisalov JN. Evaluation of caraway essential oil from different production areas of Serbia. Hort Sci (Prague) 2014; 41:122-30.

Fernández LF, Palomino OM, Frutos G. Effectiveness of Rosmarinus officinalis essential oil as antihypotensive agent in primary hypotensive patients and its influence on health-related quality of life. J Ethnopharmacol 2014; 151:509-16.

Msaada K, Hosni K, Taarit MB, Chahed T, Kchouk ME, Marzouk M. Changes on essential oil composition of coriander (Coriandrum sativum L.) fruits during three stages of maturity. Food Chem 2007; 102:1131-4.

Clauditz A, Resch A, Wieland KP, Peschel A, Götz F. Staphyloxanthin plays a role in the fitness of Staphylococcus aureus and its ability to cope with oxidative stress. Infect Immun 2006; 74:4950-3.

Burt SA, Reinders RD. Antibacterial activity of selected plant essential oils against Escherichia coli O157:H7. Lett Appl Microbiol 2003; 36:162-7.

Nazzaro F, Fratianni F, De Martino L, Coppola R, De Feo V. Effect of essential oils on pathogenic bacteria. Pharmaceuticals 2013; 6:1451-74.

Grigore A, Colceru-Mihul S, Paraschiv I, Nita S, Christof R, Iuksel R et al. Chemical analysis and antimicrobial activity of indigenous medicinal species volatile oils. Rom Biotech Lett 2012; 17:7620-7.

Akrayi HFS. Effect of some plant extracts on isolated bacteria from eyelids of natural eye liner users and eye cosmetics users. J App Pharm Sci 2012; 2:3-8.

Khalid S, Mhamed R, Mohammed B, Chakib A, Lhoussaine ER. Chemical composition and bioactivity of essential oils of seed and leaf from Foeniculum vulgare Mill. cultivated in Southeast of Morocco. J Nat Sci Res 2014; 4:33-8.

Kebede A, Hayelom M. The design and manufacturing of essential oil distillation plant for rural poverty alleviation in Ethiopia. Ethiopian J Environ Stud Manage 2008; 1:84-91.

Simic A, Rančic A, Sokovic MD, Ristic M, Grujic-Jovanovic S, Vukojevic J et al. Essential oil composition of Cymbopogon winterianus and Carum carvi and their antimicrobial activities. Pharm Biol 2008; 142:437-41.

Bosnić T, Softić D, Grujić-Vasić J. Antimicrobial activity of some essential oils and major constituents of essential oils. Acta Med Acad 2006; 35:19-22.

Fratini F, Casella S, Leonardi M, Pisseri F, Ebani VV, Pistelli L et al. Antibacterial activity of essential oils, their blends and mixtures of their main constituents against some strains supporting livestock mastitis.

Fitoterapia 2014; 96:1-7.


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