Capability of Plant Growth-Promoting Rhizobacteria (PGPR) for producing indole acetic acid (IAA) under extreme conditions
Plant Growth-Promoting Rhizobacteria (PGPR) inhabiting the area around the plant roots or in plant tissues and stimulate plant growth directly or indirectly. Synthesis of the phytohormone auxin indole-3-acetic acid (IAA) is one of the direct effects of PGPR on plant growth. This study aimed to isolate and screen IAA producing bacteria from soil and study the impacts of the alkalinity and salinity on IAA production and total antioxidant activity of the highly IAA producing strain. From the fifteen isolates tested, six were selected as efficient IAA producer, from which one isolate was highly IAA producer. The highly producing isolate was identified based on molecular characteristics using 16S rRNA. The sequence analysis showed 99% similarity with Bacillus subtilis from GenBank data base. The strain yielded IAA in a wide range of pH (5-9), giving its maximum IAA production at pH 8. High IAA concentration was also observed in the presence of 0.5% and 1% NaCl in comparison with control (with no NaCl). Furthermore, the results indicated that, total antioxidant was increased in acidic (pH 5 and pH 6) and alkaline (pH 8) media, as well as in salinity up to 2%. This study could be stated as the prospective of IAA producing bacterial isolate in the field, as a result, using it as alternative valuable biofertilizer.
2. Chandler D, Davidson G, Grant WP, Greaves J, Tatchell GM. Microbial biopesticides for integrated crop management: an assessment of environmental and regulatory sustainability. Trends Food Sci Tech. 2008; 19: 275-283.
3. Hayat R, Ali S, Amara U, Khalid R, Ahmed I. Soil beneficial bacteria and their role in plant growth promotion: a review. Ann Microbiol. 2010; 60: 579-598.
4. Ahemad M, Khan MS. Evaluation of plant growth promoting activities of rhizobacterium Pseudomonas putida under herbicide-stress. Ann Microbiol. 2012; 62: 1531-1540.
5. Spaepen S, Vanderleyden J. Auxin and plant-microbe interactions. Cold Spring Harb Perspect Biol. 2011; 3: a001438.
6. Swain MR, Naskar SK, Ray RC. Indole 3-acetic acid production and effect on sprouting of yam. (Dioscorea rotundata L.) Minisetts by Bacillus subtilis isolated from culturable cowdung microflora. Pol J Microbiol. 2007; 56: 103-110.
7. Kosuge T, Sanger M. Indole acetic acid, its synthesis and regulation: basis for tumorigen city in plant disease. Recent Adv Phytochem. 1987; 20: 147-161.
8. Oberhansli T, Defago G, Haas D. Indole-3-acetic acid (IAA) synthesis in the biocontrol strain CHAO of Pseudomonas fluorescens: role of tryptophan side chain oxidase. J Gen Microbial. 1991; 137: 2273-2279.
9. Costacurta A, Vanderleyden J. Synthesis of phytohormones by plant associated bacteria. Crit Rev Microbiol. 1995; 21: 1-18.
10. Nghia NK, Tien TT, Oanh NK, Nuong NK. Isolation and characterization of indole acetic acid producing halophilic bacteria from salt affected soil of Rice-Shrimp farming system in the Mekong Delta, Vietnam. Agric For Fish. 2017; 6(3): 69-77.
11. Schweder T, Lindequist U, Lalk M. Screening for new metabolites from marine microorganisms. Adv Biochem Engin Biotechnol. 2005; 96: 1-48.
12. Janardhan A, Kumar AP, Viswanath B, Saigopal DV, Narasimha G. Production of bioactive compounds by actinomycetes and their antioxidant properties. Biotechnol Res Int. 2014; ID 217030.
13. Al Hassan M, Chaura J, Donat-Torres M, Boscaia M, Vicenta O. Antioxidant response under salinity and drought in three closely related wild monocots with different ecological optima. AoB Plants 2017; 9: Plx 009.
14. Kumari A, Das P, Parida AK, Agarwal PK. Proteomics, metabolomics and ionomics perspectives of salinity tolerance in halophytes. Frontiers Plant Sci. 2015; 6: 537.
15. Bose J, Rodrigo-moreno A, Shabala S. ROS homeostasis in halophytes in the context of salinity stress tolerance. J Exp Bot 2014; 65: 1241-1257.
16. Sharma SK, Gupta VK. In vitro antioxidant studies of Ficus racemosa Linn. root. Pharmacognosy Mag. 2008; 13: 70-74.
17. Saha MR, Alam MA, Akter R, Jahangir R. In-vitro free radical scavenging activity of Ixora coccinea L. Bangl J Pharmacol. 2008; 3: 90-96.
18. Selim MS, Mohamed SS, Shimaa RH, El Awady ME, El Sayed OH. Screening of bacterial antioxidant exopolysaccharides isolated from Egyptian habitats. J Chem Pharm Res. 2015; 7(4): 980-986.
19. Gaur AC. Phosphate solubilizing microorganisms as biofertilizers. Omega Scientific Publishers, New Delhi, 1990.
20. Sawar M, Kremer RJ. Determination of bacterially derived auxins using microplate method. Lett Appl Microbiol. 1995; 20: 282-285.
21. Brick JM, Bostock RM, Silverstone SE. Rapid in situ assay for indole acetic acid production by bacteria immobilized on nitrocellulose membrane. Appl Environ Microbiol. 1991; 57: 535-538.
22. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999; 269(2): 337-341.
23. Williams and Twine. Flame photometric method for sodium, potassium and calcium. In: Tracey MV, edr. Modern method of plant analysis. Berlin: Springer-Verlag, 1960.
24. Yousef N. Characterization and antimicrobial activity of silver nanoparticles synthesized by rice straw- utilizing bacterium (Lysinibacillus fusiformis). Int J Develop Res. 2014; 4(9): 1875-1879.
25. Hoagland DR, Arnon DI. The water culture method for growing plants without soil. California Agr Exp Sta Cir. 1938: 337.
26. Chagas Jr AF, de Oliveira AG, de Oliveira LA, dos Santos GR, Chagas LFB, Lopes da Silva AL, da Luz Costa J. Production of indole-3-acetic acid by Bacillus isolated from different soils. Bulg J Agric Sci. 2015; 21: 282-287.
27. Araujo F, Henning A, Hungria M. Phytohormones and antibiotics produced by Bacillus subtilis and their effects on seed pathogenic fungi and on soybean root development. World J Microbiol Biotechnol. 2005; 21: 1639-1645.
28. Mirza MS, Ahmad W, Latif F, Haurat J, Bally R, Normand P, Malik KA. Isolation, partial characterization, and the effect of plant growth-promoting bacteria (PGPB) on micro-propagated sugarcane in vitro. Plant Soil. 2001; 237: 47-54.
29. Miller RL, Tegen I, Perlwitz JP. Surface radiative forcing by soil dust aerosols and the hydrologic cycle. J Geophys Res. 2004; 109, D04203.
30. Zaidi S, Usmani S, Singh BR, Musarrat J. Significance of Bacillus subtilis strain SJ 101 as a bioinoculant for concurrent plant growth promotion and nickel accumulation in Brassica juncea. Chemosphere. 2006; 64: 991-997.
31. Reetha S, Bhuvaneswari G, Thamizhiniyan P, Ravi Mycin T. Isolation of Indole acetic acid (IAA) producing rhizobacteria of Pseudomonas fluorescens and Bacillus subtilis and enhance growth of onion (Allium cepa L.). Int Curr Microbiol App Sci. 2014; 3(2): 268-574.
32. Egamberdiyeva D. Alleviation of salt stress by plant growth regulators and IAA producing bacteria in wheat. Acta Physiol Plant. 2009; 31: 861-864.
33. Nakbanpote AW, Panitlurtumpaia N, Sangdeea A, Sakulponea N, Sirisoma P, Pimthong A. Salt-tolerant and plant growth promoting bacteria isolated from Zn/Cd contaminated soil: identification and effect on rice under saline conditions. J Plant Interact. 2013; 32: 37-41.
34. Nadeem SM, Ahmad M, Naveed M, Imran M, Zahir ZA. Crowley relationship between in vitro characterization and comparative efficacy of plant growth-promoting rhizobacteria for improving cucumber salt tolerance. Arch Microbiol. 2016; 198(4): 379-387.
35. El-Shabrawi H, Kumar B, Kaul T, Reddy MK, Singla-Pareek SL, Spory SK. Redox homeostasis, antioxidant defence and methylglyoxal detoxification as markers for salt tolerance in Pokkali rice. Protoplasma. 2010; 245(1): 85-96.
36. Hu JF, Gen MY, Zhang JT, Jiang HD. An in vitro study of the structure-activity relationships of sulfated polysaccharide from brown algae to its antioxidant effect. J Asian Nat Prod Res. 2013; 4: 353-358.
37. Vurukonda SS, Vardharajula S, Shrivastava M, Skz A. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiol Res. 2016; 184: 13-24.
38. Mandal SK, Mondal KC, Dey S, Pati BR. Optimization of cultural and nutritional conditions for indole-3-acetic (IAA) production by a Rhizobium sp. Isolated from root nodules of Vigna mungo (L.). Helpper Res J Microbiol. 2007; 2: 239-246.
39. Khamna S, Yokota A, Peberdy JF, Lumyong S. Indole-3acetic acid production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils. Eur Asia J BioSci. 2010; 4: 23-32.
40. Acuna JJ, Jorquera MA, Martinez OA, Menezes-Blackburn D, Fernandez MT, Marschner P, et al. Indole acetic acid and phytase activity produced by rhizosphere Bacilli as affected by pH and metals. J Soil Sci Plant Nutr. 2011; 11: 1-12.
41. Wahyudi AT, Astuti RP, Widyawati A, Meryandini A, Nawangsih AA. Characterization of Bacillus sp. strains isolated from rhizosphere of soybean plants for their use as potential plant growth for promoting Rhizobacteria. J Microbiol Antimicrobials. 2011; 3: 34-40.
42. Mena-Violante HG, Olalde-Portugal V. Alteration of tomato fruit quality by root inoculation with plant growth-promoting rhizobacteria (PGPR): Bacillus subtilis BEB-13bs. Scientia Horticult. 2007; 113: 103-106.
43. Fatima Z, Saleemi M, Zia M, Sultan T, Aslam M, Riaz-ur-Rehman, Chaudhary MF. Antifungal activity of plant growth-promoting rhizobacteria isolates against Rhizoctonia solani in wheat. Afr J Biotechnol. 2009; 8: 219-225.
44. Ghosh S, Penterman JN, Little RD, Chavez, Glick BR. Three newly isolated plant growth-promoting bacilli facilitate the seeding of canola, Brassica campestris. Plant Physol Biochem. 2003; 41: 277-281.
45. Yousef N, Hussein NA. Impacts of inoculation with Rhizobium leguminosarum and arbuscular mycorhizae fungi and phosphate on faba bean (Vicia faba L.) grown in soil under salt stress condition. Bangl J Bot. 2017; 46(2): 599-605.
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