In vitro regeneration of plantlets from nodal explants of Aristolochia saccata and Aristolochia cathcartii

Bhaskar Sarma, Bhaben Tanti


In vitro propagation of Aristolochia saccata and A. cathcartii were carried out using nodal explant. In both the plants, nodal explants showed direct somatic embryogenesis when cultured on MS medium using various concentrations of BAP (1.0-4.0) and 2iP (1.0-4.0) separately or in combination with low concentration (0.5 and 1.0 mg l-1) of auxin (NAA). It was observed that BAP in combination with NAA was more effective for shoot induction than the hormones used separately. Among different combinations of NAA and BAP, 3.0 mg/l BAP + 1.0 mg/l NAA showed better response in case of A. saccata of about 96%, whereas, in A. cathcartii, the best response was achieved in 4.0 mg/l BAP + 0.5 mg/l NAA after 28 day of culture and 88.3% explants showed proliferation in this combination. The auxins NAA and IBA were used singly to induce rooting from in vitro raised shoot lets. A range of concentration was tested (0.1, 0.5, 0.8 and 1.0 mg/l) for rooting. In the present study 1/2 strength MS basal medium and the two different auxins (NAA and IBA) were tried, the maximum results on rooting were obtained on half strength with IBA (0.5 mg/l) then NAA.


Aristolochia saccata; A. cathcartii; In vitro regeneration; Nodal culture; MS medium

Full Text:



Srivastava J, Lambert J, Vietmeyer N. Medicinal plants: an expanding role in development, World Bank technical paper no. 320, Washington, DC: World Bank Agriculture and Forestry Systems, 1995.

Che CT, Almed MS, Kang SS, Waller DP, Bengel AS, Martin A, et al. Studies on Aristolochia III. Isolation and biological evaluation of constituents of Aristolochia indica roots for fertility regulating activity. J Nat Prod. 1984; 47: 331-341.

Das R, Kausik A, Pal TK. Anti-inflammatory activity study of antidote Aristolochia indica to the venom of Heteropneustes fossilis in rats. J Chem Pharm Res. 2010; 2: 554-562.

Sarma B, Tanti B. Karyomorphology of three species of Aristolochia - rare and endemic medicinal plants of Assam, India. Int J Cytol Cytosyst Cytogen. 2015; 68: 154-158.

Neinhuis C, Wanke S, Hilu KW, Müller K, Borsch T. Phylogeny of Aristolochia ceae based on parsimony, likelihood and Bayesian analyses of trnL-trnF sequences. Plant Syst Evol. 2005; 250: 7-26.

Wanke S, Gonzales F, Neinhuis C. Systematics of Pipevines: combining morphological and fast-evolving molecular characters to investigate the relationships within subfamily Aristolochioideae (Aristolochiaceae). Int J Plant Sci. 2006; 167: 1215-1227.

Wanke S, Jaramillo MA, Borsch T, Samain MS, Quandt D, Neinhuis C. Evolution of Piperales - matK gene and trnK intron sequence data reveal lineage specific resolution contrast. Mol Phylogenet Evol. 2007; 42: 477-497.

Wu TS, Tsai YL, Damu AG, Kuo PC, Wu PL. Constituents from the root and stem of Aristolochia elegans. J Nat Prod. 2002; 65: 1522-1525.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962; 15: 473-497.

Joshi M, Dhar U. In vitro propagation of Saussurea obvallata (DC.) Edgew. - an endangered ethno religious medicinal herb of Himalaya. Plant Cell Reports. 2003; 21: 933-939.

Martin KP. Rapid propagation of Holostemma ada-kodien Schult., a rare medicinal plant through axillary bud multiplication and indirect organogenesis. Plant Cell Reports. 2002; 21: 112-117.

Sharma N, Chandel KPS, Paul A. In vitro propagation of Gentiana kurroo - an indigenous threatened plant of medicinal importance. Plant Cell Tissue Organ Cult. 1993; 34: 307-309.

Remeshree AB, Hariharan M, Unnikrishana K. Micropropagation and callus induction of Aristolochia bracteolate Lam. a medicinal plant. Phytol. 1994; 44: 247-252.

Sultana S, Handique PJ. Micropropagation of Wedelia chinensis through high frequency shoot multiplication using nodal explants. J Curr Sci. 2004; 5: 447-452.

Chandramu C, Rao DM, Reddy DM. High frequency induction of multiple shoots from nodal explants of Vitex negundu L. using sodium sulphate. J Plant Biotech. 2003; 5: 107-113.

Sudha CG, Krishnan PN, Pushpangadan P. In vitro propagation of Holostemma annulare (Roxb.) K. Schum, a rare medicinal plant. In Vitro Cell Dev Biol Bio. Pl. 1998; 33: 57-63.

Chen Y, Hausner G, Kenaschuk E, Procunier D, Dribnenki P, Chandra G, Bhanja P. Study of organogenesis in vitro from callus tissue of Flacourtia jangomas (Lour.) Raeusch through scanning electron microscopy. Curr Sci. 2002; 83: 476-479.

Rout GR, Samantray S, Das P. In vitro manipulation and propagation of medicinal plants. Biotechnol Adv. 2000; 18: 91-120.

Sahaya-Sathish S, Janakiraman N, Johnson M. In vitro propagation of Aristolochia bracteata Retz. - a medicinally important plant. Res Biotechnol. 2011; 2: 44-52.

Chandra I, Bhanja P. Study of organogenesis in vitro from callus tissue of Flacourtia jangomas (Lour.) Raeusch through scanning electron mircroscopy. Curr Sci. 2002; 83: 476-479.

Beegum AS, Martin KP, Chun-Lai Z, Nishitha I, Ligimol K, Slater A, Madhusoodanan PV. Organogenesis from leaf and internode explants of Ophiorrhiza prostrata, an anticancer drug (camptothecin) producing plant. Electronic J Biotechnol. 2007; 10: 114-123.

Kabir AH, Mahfuz I, Razvy MA, Ahmed MB, Alam MF. Indirect organogenesis and somaclonal variation in four rice cultivars of Bangladesh. J Appl Sci Res. 2008; 4: 451-458.

Naika HR, Krishna V. Micropropagation, isolation and characterization of berberine form leaves of Naravelia zeylanica (L.) DC. Res J Med Plant. 2008; 2: 1-9.

Chamandoosti F. The relationship between plant growth regualtors for organogenesis and phenolic compound in cotton (Gossypium hirsutum L.). Asian J Develop Biol. 2010; 2: 16-22.

Kim JS, Lee SY, Eom SH, Park SU. Improved shoot organogenesis and plant regeneration of Echinacea angustifolia DC. J Med Plants Res. 2010; 4: 587-591.

Biswas A, Bari MA, Roy M, Bhadra SK. In vitro regeneration of Aristolochia tagala Champ. - a rare medicinal plant of Chittagong hill tracts. J Bio Sci Res. 2007; 15: 63-67.

Chandraprabha A, Subbu RR. Micropropagation of Aristolochia tagala Cham. - a rare and endemic medicinal plant from Western Ghats. J Bio Sci Res. 2010; 1: 70-73.

Sebastinraj J, Sidique KMI. In vitro rapid clonal propagation of Aristolochia bracteolata Lam. (Aristolochiaceae) - a valuable medicinal plant. World J Agric Sci. 2011; 7: 653-658.

Shin JH, Kim SK, Lee JB, Bong-Ho K, Shon JK. Factors affecting the production of in vitro plants from the nodal pieces of Chinese Yam (Dioscorea opposit Thunb). J Plant Biotechnol. 2004; 6: 97-102.

Ohyama K. Tissue culture in mulberry tree. Jpn Agric Res Quart. 1970; 5: 30-34.

Behera KK, Sahoo S, Prusti AB. Effect of plant growth regulator on in-vitro micropropagation of Bitter Yam (Dioscorea hispida Dennst.). Int J Integr Biol. 2008; 4: 50-54.


  • There are currently no refbacks.