Influence of extracellular matrix on the proliferation and adhesion properties of stem cells derived from different sources

Anna Bajek, Dorota Porowińska, Krzysztof Roszkowski

Abstract


One of the most important issues in regenerative medicine is the development of culture conditions mimicking the natural ones, which allows obtaining a large number of cells and their long-term maintenance in undifferentiated state. In vivo, cells are surrounded by a specific microenvironment called extracellular matrix (ECM), which plays an important role in the regulation of processes such as proliferation, migration, differentiation or apoptosis. In this study we assessed the influence of different extracellular matrix components (fibronectin, laminin, collagen IV, poly-D-lysine) on the in vitro adhesion and proliferation of stem cells isolated from bone marrow, adipose tissue and hair follicles. Our results showed that stem cells derived from different sources present various responses to ECM components. None of the tested extracellular proteins reduced the proliferation of bone marrow as well as adipose-derived mesenchymal stem cells, with the exception of laminin. This demonstrates the biocompatibility of such modified surfaces and possibility of using them for culturing these types of stem cells. Different results were obtained for hair follicle stem cells. The presented results indicate that ECM is an important component of the cellular niche in the tissue. It is also possible that ECM is required for the reconstitution of the niche of stem cells in vitro.

Keywords


Stem cells; Bone marrow; Adipose tissue; Hair follicles; Extracellular matrix

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References


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