In vitro studies of iron absorption and activity of glutathione peroxidase in intestinal mucosa of the chicken
Keywords:
Glutathione peroxidase, Iron absorption, Intestinal mucosa, Chicken
Abstract
We examined the absorption of iron, the activity of selenoprotein glutathione peroxidase (GSH·Px) and cellular compartmentalization of metal in the chicken duodenum and ileum. The method of accumulating mucosa preparation (AMP) was used. It was shown that the intestinal iron accumulation is dose-dependent process, which has two components: transcellular and paracellular. The realization of these pathways is region-specific and depends on exposed iron levels. Slightly elevated iron status of intestinal mucosa does not influence activity of GSH·Px. At the same time the results indicate that the activity of glutathione peroxidase can be altered by iron overload. Immunohistochemistry revealed that stainable iron could be co-localized to the endolysosomal compartment. How the activity of enzyme can be affected by oxidative stress and competitive interactions of iron with selenium are discussed.
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References
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2. Dixon SJ, Stockwell BR. The role of iron and reactive oxygen species in cell death. Nat Chem Biol. 2014; 10: 9-17.
3. Galaris D, Pantopoulos K. Oxidative stress and iron homeostasis: mechanistic and health aspects. Crit Rev Clin Lab Sci. 2008; 45(1): 1-23.
4. Genc GE, Ozturk Z, Gumuslu S. Selenoproteins are involved in antioxidant defense systems in thalassemia. Metallomics. 2017; 9: 1241-1250.
5. Baker RD, Baker SS, LaRosa K, Whitney C, Newburger PE. Selenium regulation of glutathione peroxidase in human hepatoma cell line Hep3B. Arch Biochem Biophys. 1993; 304(1): 53-57.
6. Spears JW. Trace mineral bioavailability in ruminants. J Nutr. 2003; 33(5): 1506S-1509S.
7. Oates PS, Thomas C. Augmented internalisation of ferroportin to late endosomes impairs iron uptake by enterocyte-like IEC-6 cells. Pflugers Arch. 2005; 450(5): 317-325.
8. Bresgen N, Eckl PM. Oxidative stress and the homeodynamics of iron metabolism. Biomolecules. 2015; 5(2): 808-847.
9. Lönnerdal B. Alternative pathways for absorption of iron from foods. Pure Appl Chem. 2010; 82(2): 429-436.
10. Carmona-Gutierrez D, Hughes AL, Madeo F, Ruckenstuhl C. The crucial impact of lysosomes in aging and longevity. Age Res Rev. 2016; 32: 2-12.
11. Close B, Banister K, Baumans V, Bernoth EM, Bromage N, Bunyan J, et al. Recommendations for euthanasia of experimental animals Part 1. Lab Animals. 1996; 30(4): 293-316.
12. Anonymus. Analytical methods for atomic absorption spectrophotometry. The Perkin-Elmer Corporation, Norwalk, 1971.
13. Pinto R, Bartley W. The effect of age and sex on glutathione reductase and glutathione peroxidase activities and aerobic glutathione oxidation in rat liver homogenates. Biochem J. 1969; 112(1): 109-115.
14. Ugolev A, Zhigure D, Nurks J. Accumulating mucosal preparation - a new method in the initial stages of substance transport across the intestinal wall. Physiol J USSR. 1970; 56: 1638-1641.
15. Sáiz MP, Martí MT, Mitjavila MT, Planas J. Iron absorption by small intestine of chickens. Biol Trace Elem Res. 1993; 36(1): 7-14.
16. Bronner F. Mechanisms of intestinal calcium absorption. J Cell Biochem. 2003; 88(2): 387-393.
17. Skjørringe T, Burkhart A, Johnsen KB, Moos T. Divalent metal transporter 1 (DMT1) in the brain: implications for a role in iron transport at the blood-brain barrier, and neuronal and glial pathology. Front Mol Neurosci. 2015; 8: 19.
18. Saito M, Hanson PI, Schlesinger P. Luminal chloride-dependent activation of endosome calcium channels: patch clamp study of enlarged endosomes. J Biol Chem. 2007; 282(37): 27327-27333.
19. Miyamoto Y, Koh YH, Park YS, Fujiwara N, Sakiyama H, Misonou Y, et al. Oxidative stress caused by inactivation of glutathione peroxidase and adaptive responses. Biol Chem. 2003; 384(4): 567-574.
20. Winterbourn CC. Toxicity of iron and hydrogen peroxide: the Fenton reaction. Toxicol Lett. 1995; 82-83: 969-974.
21. Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv. 2015; 5: 27986-28006.
22. Viita LM, Mutanen ML, Mykkanen HM. Selenium-iron interaction in young women with low selenium status. J Nutr Diet. 1989; 2(1): 39-42.
23. Linder MC, Moriya M, Whon A, Kassa A, Gilley C. Vesicular transport of fe and interaction with other metal ions in polarized Caco2 cell monolayers. Biol Res. 2006; 39(1): 143-156.
24. Núñez MT. Regulatory mechanisms of intestinal iron absorption-uncovering of a fast-response mechanism based on DMT1 and ferroportin endocytosis. Biofactors. 2010; 36(2): 88-97.
25. Hong CH, Falvey C, Harris TB, Simonsick EM, Satterfield S, Ferrucci L, et al. Anemia and risk of dementia in older adults: findings from the Health ABC study. Neurology. 2013; 81(6): 528-533.
26. Killilea D, Atamna H, Liao B. Iron accumulation during cellular senescence in human fibroblasts in vitro. Antioxid Redox Signal. 2003; 5(5): 507-516.
27. Xu J, Knutson M, Carter C, Leeuwenburgh C. Iron accumulation with age, oxidative stress and functional decline. PLoS One. 2008; 3(8): e2865.
28. Altamura S, Muckenthaler MU. Iron toxicity in diseases of aging. J Alzheimer’s Dis. 2009; 16(4): 879-895.
29. Ong W, Jenner A, Pan N, Ong C, Halliwell B. Elevated oxidative stress, iron accumulation around microvessels and increased 4-hydroxynonenal immunostaining in zone 1 of the liver acinus in hypercholesterolemic rabbits. Free Radic Res. 2009; 43(3): 241-249.
30. Klipstein-Grobusch K, Koster J, Grobbee D. Serum ferritin and risk of myocardial infarction in the elderly: the Rotterdam Study. Am J Clin Nutr. 1999; 69(6): 1231-1236.
31. Bhasin G, Kausar H, Sarwar Alam M, Athar M. Progressive iron overload enhances chemically mediated tumor promotion in murine skin. Arch Biochem Biophys. 2003; 409(2): 262-273.
Published
2018-09-10
How to Cite
(1)
Markovs, J.; Galuza, A.; Basova, N.; Knipse, G.; Vasiljeva, S.; Smirnova, G. In Vitro Studies of Iron Absorption and Activity of Glutathione Peroxidase in Intestinal Mucosa of the Chicken. European Journal of Biological Research 2018, 8, 168-173.
Section
Research Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
