Immunosuppressive and cytotoxic potential of aqueous leaves extract of Thlaspi arvense

Amit Gupta, Bharat Shinde


Medicinal plants are traditionally used in Asian countries for treatment of various intracellular as well as extracellular infections. The present study was undertaken to investigate Thlaspi arvense for determining its immunosuppressive potential and cytotoxic activity in infected lysed human whole blood samples. For these studies, virally infected human whole blood samples (n = 5; symptoms such as sneezing, cough etc.) were collected and analysing its cytotoxicity of variable doses of aqueous leaves extract of Thlaspi arvense in the form of proliferation using Concanavalin (Con A; 2.5 µg/ml). In addition, immunosuppressive activity was also determined using lysed human whole blood samples were incubated with variable doses of aqueous extract in presence or absence of incomplete freunds adjuvant (IFA) pertaining to determine the total cellular (protein) content. In contrast, in vitro studies of aqueous extract of Thlaspi arvense were also determined using lactoferrin (100 µg/well) and ovalbumin (OVA, 100 µg/well) as coating antigen pertaining to antibody production using Elisa. The results showed that aqueous leaves extract of Thlaspi arvense showed markedly decline in Con A proliferation at higher doses. In addition, this aqueous extract also showed decline in total cellular content in presence or absence of IFA but it showed enhancement in antibody production in vitro against lactoferrin and OVA at higher doses. Overall the data showed that aqueous leaves extract of Thlaspi arvense displayed immunosuppressive and cytotoxic effect in virally infected human whole blood samples.


Thlaspi arvense; Cytotoxic; Immunosuppressive; Complete freunds adjuvant

Full Text:



Gupta A, Chaphalkar SR. Development of novel plant based adjuvant formulation against rubella and hepatitis B vaccine antigen. Herba Polonica. 2016; 62(3): 40-48.

Gupta A, Chaphalkar SR. Anti-inflammatory and anti-microbial activities of aqueous leaves extract of Butea frondosa. J Herb Med Pharmacol. 2016; 5(2): 7-11.

Gupta A, Chaphalkar SR. Cytotoxic and antiviral activity of Acacia catechu on human peripheral blood mononuclear cells. Indonesian J Pharmacy. 2016; 27(2): 111-116.

Gupta A, Khamkar PR, Chaphalkar SR. Applications and uses of active ingredients from medicinal plants. Indian J Novel Drug Deliv. 2014; 6(2): 106-111.

Khajuria A, Gupta A, Singh S, Malik F, Singh J, Suri KA, et al. RLJ-NE-299A: a new plant based vaccine adjuvant. Vaccine. 2007; 25(14): 2706-2715.

Gupta A, Khamkar PR, Chaphalkar SR. Review on medicinal plants to target and inhibit the epidermal growth factor receptor signaling in cancer and tissue repair therapy. Int J Adv Pharm Biol Chem. 2014; 3(1): 210- 213.

Golden RN, Peterson F. The truth about illness and disease. Infobase Publishing, 2009.

Deirdre BJ, Ian MG. Maximizing the impact of antimicrobial stewardship. Curr Opin Infect Dis. 2013; 26(4): 352-358.

Heffernan TM. The impact of excessive alcohol use on prospective memory: a brief review. Curr Drug Abuse Rev. 2008; 1(1): 36-41.

Pedras MSC, Chumala PB, Suchy M. Phytoalexins from Thlaspi arvense, a wild crucifer resistant to virulent Leptosphaeria maculans: structures, syntheses and antifungal activity. Phytochem. 2003; 64: 949-955.

Moser BR, Shah SN, Winkler-Moser JK, Vaughn SF, Evangelista RL. Composition and physical properties of cress (Lepidium sativum L.) and field pennycress (Thlaspi arvense L.) oils. Ind Crops Prod. 2009; 30: 199-205.

Walter NS, Bagai U. Antiplasmodial potential of traditional medicinal plant Thlaspi arvense. Eur J Med Plants. 2014; 4(12): 1378-1387.

Sedbrook JC, Phippen WB, Marks MD. New approaches to facilitate rapid domestication of a wild plant to an oilseed crop: example pennycress (Thlaspi arvense L.). Plant Sci. 2014; 22(7C): 122-132.

Dorn KM, Fankhauser JD, Wyse DL, Marks MD. De novo assembly of the pennycress (Thlaspi arvense) transcriptome provides tools for the development of a winter cover crop and biodiesel feedstock. Plant J. 2013; 75: 1028-1038.

Gupta A, Shah AP, Chabukswar AR, Chaphalkar SR. Extraction of proteases from leaves of Mimusops elengi and its immunopharmacological applications. Indo Am J Pharmac Sci. 2016; 3(3): 211-220.

Shaikh AC, Gupta A, Chaphalkar SR. Quantitative phytochemical credential of Nakshtra plants and distribution analysis of aldehydes pertaining to LC-MS. Int J Chem Pharmac Sci. 2016; 4(8): 267-274.

Gupta A, Chaphalkar SR. Inhibition of antigen specific T cell population using Calotropis gigantea and Terminalia arjuna. J Biol Nature. 2016; 5(1): 14-19.

Gupta A, Chaphalkar SR. Immunoadjuvant potential of Azadirachta indica against rabies, hepatitis and DPT vaccine antigen. Int J Med Pharmac Sci. 2015; 5(7): 1-5.

Gillett A, Chan C. Applications of immunohistochemistry in the evaluation of immunosuppressive agents. Human Exp Toxicol. 2000; 19(4): 251-254.

Wei L, Mac Donald TM, Walker BR. Taking glucocorticoids by prescription is associated with subsequent cardiovascular disease. Ann Intern Med. 2004; 141: 764-770.

Vonkeman HE, van de Laar MA. Nonsteroidal anti-inflammatory drugs: adverse effects and their prevention. Semin Arthritis Rheum. 2010; 39: 294-312.

Smarr CB, Bryce PJ, Miller SD. Antigen-specific tolerance in immunotherapy of Th2-associated allergic diseases. Crit Rev Immunol. 2013; 33(5): 389-414.

Huszarik K, Wright B, Keller C, Nikoopour E. Adjuvant immunotherapy increases beta cell regenerative factor Reg2 in the pancreas of diabetic mice. J Immunol. 2010; 185(9): 5120-5129.

Gupta A, Chaphalkar SR. Vaccine adjuvants: the current necessity of life. Shiraz E-Med J. 2015; 16(7): e28061.


  • There are currently no refbacks.