Phytochemical profile, total phenolic content and antioxidant activity of ethanolic extract of fumitory (Fumaria capreolata L.) from Algeria
Keywords:
Fumaria capreolata L., Phytochemical screening, Antioxidant activity, DPPH, β-carotene, FRAP
Abstract
Fumitory or Fumaria capreolata L. is a medicinal plant, spontaneous and widely distributed in North Africa, particularly in Algeria. It has been recognized for centuries for its therapeutic virtues, and it is used in traditional medicine in the treatment of hepatobiliary diseases, gastrointestinal disorders and in the treatment of skin diseases. The phytochemical screening carried out on the aerial part of the species F. capreolata L., revealed the richness of this plant in secondary metabolites, such as alkaloids, catechic tannins, sterols and terpenes. On the other hand, we noticed the absence of cardinolides, leuco-anthocyanins, quinones and starch in all parts of the plant. Quantitative spectrophotometric analysis allowed us to detect the levels of total polyphenols using the reagent of Folin-Ciocalteu, according to the results obtained we find that the species F. capreolata is rich in these compounds (14.27 ± 1.65 mg GAE/g). The evaluation of the antioxidant activity was carried out using the DPPH method, indicated that the ethanolic extract of F. capreolata L. showed significant antioxidant activity, with an IC50 = 0.27 mg/ml. And it also has a strong inhibitory activity of the coupled oxidation of linoleic acid and β-carotene, with a percentage of 88.46 ± 1.02% at a concentration of 0.5 mg/ml. In addition, the crude extract of F. capreolata L., also exhibits a good iron reduction capacity, with a maximum optical density of 0.349 at a concentration of 0.5 mg/ml.
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References
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34. Bribi N, Bouguezza Y, Maiza BF. Evaluation of erythrocytes toxicity and antioxidant activity of alkaloids of Fumaria capreolata. Int J Pharma Bio Sci. 2013; 2: 770-776.
35. Orhan I, Sener B, Choudhary MI, Khalid A. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. J Ethnopharmacol. 2004; 57-60.
36. Moghaddam M, Seyed Naser Khaleghi Miran SN, Mehdizadeh L. Total phenolic content and antioxidant activity of Fumaria vaillantii extract at three phenological stages assayed by various methods. Int J Hortic Sci. 2018; 5(1): 93-102.
37. HabibiTirtash F, Keshavarzi M, Fazeli F. Antioxidant components of Fumaria species. World Acad Sci Engin Technol. 2011; 74: 238-241.
38. Kaur C, Kapoor HC. Antioxidant activity and total phenolic content of some Asian vegetables. Food Sci Technol. 2002; 37: 153-161.
39. Yang J, Guo J, Yuan J. In vitro antioxidant properties of rutin. LWT. 2008; 41: 1060-1066.
40. Rosa LA, Alvarez PE, Gonzalez GA. Fruit and vegetabale phytochemicals: Chemistry, Nutritional Value and Stability. John Wiley and Sons. 2009.
41. Bourgou S, Ksouri R, Bellila A, Skandrani I, Falleh H, Marzouk B. Phenolic composition and biological activities of Tunisian Nigella sativa L shoots and roots. C. R. Biologies. 2008; 48-55.
2. Rice-Evans CA, Packer L. Flavonoids in Health and Disease, 2nd edn. CRC Press. 2003.
3. Quezel P, Santa S. La nouvelle flore de l’Algérie et des régions désertiques méridionales. Ed. Centre national de la recherche. Paris. 1963.
4. Ngakegni-Limbili AC. Etude de synergie des effets chimiques et biologiques des lipides de réserves et des huiles essentielles des fruits et graines saisonniers de la sous-région Afrique Centrale. Institut National Polytechnique. Université de Toulouse. 2012; 170-171.
5. Solfo R. Etude d’une Plante Médicinale Malgache Buxusma dagascarica Bail et ses variétés. Ed. O.R.S.T.O.M. 1973.
6. Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. Chapman et Hall Thomson Science (UK). 1998.
7. Rehman S, Abdul L, Shamim A, Asad UK. Antibacterial activity of Shahtra (Fumaia officinales Linn) extracts against MRSA (Methicillin Resistant Staphyloccocus aureus). Unani Medicus Int J. 2010; 1: 57-61.
8. Juntachote T, Berghofer E, Siebenhandl S, Bauer F. The ant-oxidative properties of Holy basil and Galangal in cooked ground pork. Meat Sci. 2006; 72: 446-456.
9. Benhammou N, Atik-Bekkara F, Kadifkova PT. Antiradical capacity of the phenolic compounds of Pistacia lentiscus L. and Pistacia atlantica Desf. Adv Food Sci. 2007; 155-161.
10. Ozsoy N, Can A, Yanardag R, Akev A. Antioxidant activity of Smilax excelsa L. leaf extracts. Food Chem. 2008; 571-583.
11. Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr. 1986; 413-419.
12. Sousek J, Guedon D, Adam T, Bochorakova H, Taborska E, Valka I, Šimanek V. Alkaloids and organic acids content of eight Fumaria species. Phytochem Anal. 1999; 10: 6-11.
13. Bentley KW. β-Phenylethylamines and the isoquinoline alkaloids. Royal Soc Chem. 2000; 247-268.
14. Suau R, Cabezudo B, Rico R, Najera F, Lopez RJM. Direct Determination of Alkaloid Contents in Fumaria Species by GC-MS. Phytochem Anal. 2002; 13: 363-367.
15. Maiza BF, Khentache S, Bougoffa K, Chibane M, Adach S, Chapeleur Y, et al. Antioxidant activities of alkaloid extracts of tow Algerian species of Fumaria: Fumaria capreolata and Fumaria bastardii. Rec Nat Prod. 2007; 1: 28-35.
16. Gupta P, Rao CHV. Morpho-anatomical and physicochemical studies of Fumaria indica (Hausskn.) Pugsley. Asian Pac J Trop Biomed. 2012; 830-834.
17. Shayka A, Chatterjee SS, Kumar V. Holistic psychopharmacology of Fumaria indica. Chinese Med. 2012; 3: 182-199.
18. Rao CV, Verma AR, Gupta PK, Kumar MV. Anti-Inflammatory and Anti-Nociceptive Activities of Fumaria indica Whole Plant Extract in Experimental Animals. Acta Pharm. 2007; 57(4): 491-498.
19. Gupta P, Sharma N, Rao CHV. A review on ethnobotany, phytochemistry and pharmacology of Fumaria indica (Fumitory). Asian Pac J Trop Biomed. 2012; 665-669.
20. Mezhoud K, Benmokhtar M, Smati D. Contribution to the study of Fumaria capreolata L. (Papaveraceae), in the Wilaya of Constantine: phytochemical characterization and research of the anti-inflammatory activity. Batna J Med Sci. 2018; 5(1): 26-28.
21. Pehlivan KF, Yildirim A, Türker A. Biological screening of various medicinal plant extracts for antibacterial and antitumor activities. Turk J Biol. 2012; 36: 641-652.
22. Al qasoumi SI, Al dosari MS. Evaluation of the hepatoprotective effect of Fumaria parviflora and Momordica balsamina from Saudi folk medicine against experimentally induced liver injury in rats. Res J Med Plant. 2009; 3: 9-15.
23. Mohajerani F, Pourjabbar Z, Mazdeh FZ, Rahimi R, Amin GR, Toliyat T, et al. Quantitation of phytochemical constituents of Fumaria vaillantii L. with different extract methods. Tradit Med Res. 2019; 4(5): 237-245.
24. Wasu SJ, Muley BP. Antioxidant Activity of Fumaria officinalis Linn and Its study on ethanol induced-immuno-supression. Res J Pharm Tech. 2009; 2: 405- 408.
25. Pandey GG, Kumar GR, Gupta ShS, Ojha SK, Rao Ch V. Wound Repair and Anti-inflammatory Potential of Fumaria indica in Excision Wound-induced Rats. Br J Pharm Res. 2014; 4: 257-266.
26. Duraffourd C, Lapraz JC. Traité de phytothérapie Clinique: endobiogénine et médecine. Elsevier Masson, 2002.
27. Naz I, Palomares-Rius JE, Saifullah, Blok V, Khan MR, Ali S. In vitro and in planta nematicidal activity of Fumaria parviflora (Fumariaceae) against the southern root-knot nematode Meloidogyne incognita. Plant Pathol. 2013; 62: 943-952.
28. Guna G. Pharmacological activity of Fumaria indica, A review. J Phytopharm. 2017; 6(6): 352-355.
29. Waston RR. Polyphenols in Plants: Isolation, Purification and Extract Preparation. Academic Press, 2014.
30. Paltinean R, Mocan A, Vlase L, Gheldiu AM, Crisan G, Ielciu I, et al. Evaluation of polyphenolic content, antioxidant and diuretic activities of six Fumaria species. Molecules. 2017; 22 (639): 1-14.
31. Ivan GI, Radka ZV, Andrey SM, Nadezhda TP, Ina YA, Panteley PD. Antioxidant activities and phenolic compounds in Bulgarian Fumaria species. Int J Curr Microbiol App Sci. 2014; 3(2): 296-306.
32. Orhan I, Sener B, Musharraf S. Antioxidant and hepatoprotective activity appraisal of four selected Fumaria species and their total phenol and flavonoid quantities. Exp Toxicol Pathol. 2010; 1-5.
33. Macheix JJ, Fleuriet A, Allemand CJ. Les composes phénoliques des végétaux: un example de metabolites secondaires d’importance économique. PPUR presses polytechniques. 2005.
34. Bribi N, Bouguezza Y, Maiza BF. Evaluation of erythrocytes toxicity and antioxidant activity of alkaloids of Fumaria capreolata. Int J Pharma Bio Sci. 2013; 2: 770-776.
35. Orhan I, Sener B, Choudhary MI, Khalid A. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. J Ethnopharmacol. 2004; 57-60.
36. Moghaddam M, Seyed Naser Khaleghi Miran SN, Mehdizadeh L. Total phenolic content and antioxidant activity of Fumaria vaillantii extract at three phenological stages assayed by various methods. Int J Hortic Sci. 2018; 5(1): 93-102.
37. HabibiTirtash F, Keshavarzi M, Fazeli F. Antioxidant components of Fumaria species. World Acad Sci Engin Technol. 2011; 74: 238-241.
38. Kaur C, Kapoor HC. Antioxidant activity and total phenolic content of some Asian vegetables. Food Sci Technol. 2002; 37: 153-161.
39. Yang J, Guo J, Yuan J. In vitro antioxidant properties of rutin. LWT. 2008; 41: 1060-1066.
40. Rosa LA, Alvarez PE, Gonzalez GA. Fruit and vegetabale phytochemicals: Chemistry, Nutritional Value and Stability. John Wiley and Sons. 2009.
41. Bourgou S, Ksouri R, Bellila A, Skandrani I, Falleh H, Marzouk B. Phenolic composition and biological activities of Tunisian Nigella sativa L shoots and roots. C. R. Biologies. 2008; 48-55.
Published
2021-09-07
How to Cite
(1)
Sofiane, I.; Seridi, R. Phytochemical Profile, Total Phenolic Content and Antioxidant Activity of Ethanolic Extract of Fumitory (Fumaria Capreolata L.) from Algeria. European Journal of Biological Research 2021, 11, 404-416.
Section
Research Articles
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