Effect of different plant bio-stimulants in improving cucumber growth under soilless culture

  • Sayed Hussein Abdelgalil Central Laboratory of Organic Agriculture (CLOA), Agricultural Research Center, 9 Gamaa Street, 12619, Giza, Egypt
  • Esraa Abdallah Horticulture Research Institute, Vegetables Cross-Pollination Department, Agricultural Research Center (ARC), Giza, Egypt
  • Weijie Jiang Institute of Vegetables and Flowers, Soilless Culture Department, Chinese Academy of Agricultural Sciences, Beijing, China
  • Basheer Noman Sallam Department of Horticulture, Faculty of Agricultural, Sana’a University, Yemen
  • Hongjun Yu Institute of Vegetables and Flowers, Soilless Culture Department, Chinese Academy of Agricultural Sciences, Beijing, China
  • Peng Liu Institute of Vegetables and Flowers, Soilless Culture Department, Chinese Academy of Agricultural Sciences, Beijing, China
Keywords: Vegetables, Greenhouse, Root system, Artificial substrate


There are more studies about plant bio-stimulants but no clear results about which is the best one in improving vegetable crops specially cucumber. The aim of this study is  to screen the effect of various bio-stimulants in improving cucumber (Cucumis sativus L.) growth under soilless culture via root application by modifying coco-peat culture media substrate. In the present study, we tested fifteen treatments as follow: T1 -control (CK); T2 - 10 mM putrescine (Put); T3 - 250 ppm seaweed (Sea); T4 - 0.02 ppm meta-topolin (MT); T5 - 100 ppm naphthalene acetic acid (NAA); T6 - 400 ppm polyaspartic acid (PAS); T7 - 50 ppm sodium nitrophenolate (98% NIT); T8 - 100 ppm tryptophan (AAF); T9 - 1% fulvic acid (FUL); T10 - 107 CFU/ml Bacillus subtilis (BAS); T11 - 106 CFU/ml Trichoderma (TRI); T12 - 50 ppm alanine (ALa); T13 - 150 ppm salicylic acid (SA); T14 - 1 mM silicon (SiO2) and T15 - 0.001 ppm 24-epibrassinolide (EBR). The results obviously showed that using all bio-stimulants significantly increased cucumber growth parameters (plant height, stem diameter, leaves number, leaf area, shoot fresh weight, and root fresh weight). Seedlings Vigor Index (SVI) increased multifold compared with control by all treatments. The increase in cucumber seedlings vigor had a highly significant effect compared with control and the increase was 55.9% followed by 55.2% and 53.4% by Put, MT, and EBR treatments respectively. Our study concluded that the application of plant bio-stimulants can be used to modify coco-peat substrate with a positive effect on plant growth and improvement of cucumber plants under soilless culture.

DOI: http://dx.doi.org/10.5281/zenodo.4420278


Download data is not yet available.


1. Peyvast G, Noorizadeh M, Hamidoghli J, Ramezani-Kharazi P. Effect of four different substrates on growth, yield and some fruit quality parameters of cucumber in bag culture. Int Sympos Growing Media. 2005; 779: 535-540.
2. Bohme M, Hoang LT, Vorwerk R. Effect of different substrates and mineral as well as organic nutrition on the growth of cucumber in closed substrate systems. Acta Hortic. 2001; DOI: 10.17660/ActaHortic.2001.548.17
3. Grafiadellis I, Mattas K, Maloupa E, Tzouramani I, Galanopoulos K. An economic analysis of soilless culture in gerbera production. Hort Sci. 2000; 35: 300-303.
4. Schiefelbein JW, Benfey PN. The development of plant roots: new approaches to underground problems. Plant Cell. 1991; 3(11): 1147-1154.
5. Verdonck O, Demeyer P. The influence of the particle sizes on the physical properties of growing media. Int Sympos Growing Media Hydroponics. 2001; 644: 99-101.
6. Suslow PD, Trevor V. Introduction to ORP as the standard of postharvest water disinfection monitoring. Veg Res Inf Cent. 1998: 1-4.
7. Bowie NE. University-Business Partnerships: An Assessment. Issues in Academic Ethics, 1994.
8. Sherlock R, Morrey JD. Ethical issues in biotechnology. Rowman & Littlefield Publishers, 2002.
9. Muscolo A, Sidari M, Nardi S. Humic substance: relationship between structure and activity. Deeper information suggests univocal findings. J Geochem Explor. 2013; 129: 57-63.
10. Chiavon M, Ertani A, Nardi S. Effects of an alfalfa protein hydrolysate on the gene expression and activity of enzymes of the tricarboxylic acid (TCA) cycle and nitrogen metabolism in Zea mays L. J Agric Food Chem. 2008; 56(24): 11800-11808.
11. Anonymous. 2013. Biostimulants market - by active ingredients applications, crop types & geography - global trends & forecasts to 2018. http://www.marketsandmarkets.com/Market-Reports/biostimulantmarket-1081.html?gclid=CJfhh9TvorgCFcU5QgodkTMApw.
12. Le Mire G, Nguyen ML, Fassotte B, du Jardin P, Verheggen F, Delaplace P, Haissam J. Implementing plant biostimulants and biocontrol strategies in the agroecological management of cultivated ecosystems. BASE. 2016; 20(1): 299-313.
13. du Jardin P. Plant biostimulants: definition, concept, main categories and regulation. Sci Hortic. 2015; 196: 3-14.
14. Basak A. Biostimulators - definitions, classification and legislation. Biostimulators Mod Agric Gen Asp. 2008: 7-17.
15. Kauffman GL, Kneivel DP, Watschke TL. Effects of a biostimulant on the heat tolerance associated with photosynthetic capacity, membrane thermostability, and polyphenol production of perennial ryegrass. Crop Sci. 2007; 47(1): 261-267.
16. Calvo P, Nelson L, Kloepper JW. Agricultural uses of plant biostimulants. Plant Soil. 2014; 383(1-2): 3-41.
17. Carrow RN, Duncan RR. Best management practices for saline and sodic turfgrass soils: assessment and reclamation. CRC Press, 2011.
18. Jadav RG, Patel TV, Parmar AB, Saiyad MY. Sex modification of cucumber vegetable through PGRs. PRAJNA. 2010; 18: 13-14.
19. Mia MAB, Islam MS, Shamsuddin ZH. Altered sex expression by plant growth regulators: An overview in medicinal vegetable bitter gourd (Momordica charantia L.). J Med Plants Res. 2014; 8: 361-367.
20. Papadopoulos AP, Saha U, Hao X, Khosla S. Response of rockwool-grown greenhouse cucumber, tomato, and pepper to kinetin foliar sprays. Hort Technol. 2006; 16(3): 493-501.
21. Abdel-Rahman M. Modification of flowering, sex expression and fruiting of selected cucurbits by growth-regulating chemicals. 1972.
22. Tantasawat PA, Sorntip A, Pornbungkerd P. Effects of exogenous application of plant growth regulators on growth, yield, and in vitro gynogenesis in cucumber. Hort Sci. 2015; 50(3): 374-382.
23. Kulkarni MG, Ascough GD, Van Staden J. Effects of foliar applications of smoke-water and a smoke-isolated butenolide on seedling growth of okra and tomato. Hort Sci. 2007; 42(1): 179-182.
24. Heckman J. Effect of an organic biostimulant on cabbage yield. J Home Consum Hortic. 1993; 1(1): 111.
25. Parađiković N, Vinković T, Vinković Vrček I, Žuntar I, Bojić M, Medić‐Šarić. Effect of natural biostimulants on yield and nutritional quality: an example of sweet yellow pepper (Capsicum annuum L.) plants. J Sci Food Agric. 2011; 91(12): 2146-2152.
26. AzconaI, Pascual I, Aguirreolea J, Fuentes M, García‐Mina JM, Sánchez‐Díaz M. Growth and development of pepper are affected by humic substances derived from composted sludge. J Plant Nutr Soil Sci. 2011; 174(6): 916-924.
27. Schiefelbein JW, Benfey PN. The development of plant roots: new approaches to underground problems. Plant Cell. 1991; 3(11): 1147-1154.
28. Du J, Shu S, An Y, Zhou H, Guo S, Sun J. Influence of exogenous spermidine on carbon–nitrogen metabolism under ca (NO3)2 stress in cucumber root. Plant Growth Regul. 2017; 81(1): 103-115.
29. Das BC, Das TK. Efficacy of GA3, NAA and Ethrel on sex expression in pumpkin (Cucurbita moschata Poir) cv. Guamala Local. Orissa J Hortic. 1995; 23(1&2): 87-91.
30. Xia XJ, Huang LF, Zhou YH, Mao WH, Shi K, Wu JX, et al. Brassinosteroids promote photosynthesis and growth by enhancing activation of Rubisco and expression of photosynthetic genes in Cucumis sativus. Planta. 2009; 230(6): 1185.
31. Solaimalai A, Sivakumar C, Anbumani S, Suresh T, Arulmurugan K. Role of plant growth regulators in rice production - a review. Agric Rev. 2001; 22(1): 33-40.
32. Zhu Y, Guo J, Feng R, Jia J, Han W, Gong H. The regulatory role of silicon on carbohydrate metabolism in Cucumis sativus L. under salt stress. Plant Soil. 2016; 406(1-2): 231-249.
33. Ertani A, Sambo P, Nicoletto C, Santagata S, Schiavon M, Nardi S. The use of organic biostimulants in hot pepper plants to help low input sustainable agriculture. Chem Biol Technol Agric. 2015; 2(1): 11.
34. Bhattacharya A, Sood P, Citovsky V. The roles of plant phenolics in defence and communication during Agrobacterium and Rhizobium infection. Mol Plant Pathol. 2010; 11(5): 705-719.
35. Yang S, Zhang Z, Cong L, Wang X, Shi S. Effect of fulvic acid on the phosphorus availability in acid soil. J Soil Sci Plant Nutr. 2013; 13(3): 526-533.
How to Cite
Abdelgalil, S.; Abdallah, E.; Jiang, W.; Sallam, B.; Yu, H.; Liu, P. Effect of Different Plant Bio-Stimulants in Improving Cucumber Growth under Soilless Culture. European Journal of Biological Research 2021, 11, 146-155.
Research Articles