L-cysteine ameliorated testicular toxicity induced by acrylamide in rats

Hossam El-Din Mohamed Omar, Sary Kh. Abd-elghafar, Imhemed O. Fiedan, Emad A. Ahmed


The general public is exposed to acrylamide from industrial manufacturing, laboratory work, foods rich in carbohydrates that have been cooked at high-temperature, and through cigarette smoke. The present experiment was conducted to investigate the reproductive toxicity of acrylamide exposure in male rats and the role of L-cysteine supplementation in amelioration of this toxicity. Forty eight adult male albino rats (weighing 120-140 g) were divided into four groups (16 rats/group). Group I - negative control group, drank tap water, group II - positive control, drank tap water that contains acrylamide (25 mg/kg body weight) for 28 days and group III drank tap water that contains acrylamide (25 mg/kg body weight) and L-cysteine (100 mg/kg body weight). Four rats from each group were killed at 7, 14, 21 and 28 days time intervals from the beginning of the experiment. In general, exposure to acrylamide induced a significant elevation in testes lipid peroxides and nitric oxide levels and a significant reduction in the level of glutathione and the activity of superoxide dismutase and catalase in all periods of experiment. However, plasma testosterone was significantly decreased in acrylamide treated rats with congestion and interstitial edema, necrosis, calcification and degeneration of spermatogenic cells in the seminiferous tubules and formation of spermatid gaint cells. Co-treatment of rats with L-cysteine reduced the changes in oxidative stress parameters and improved the pathological changes in testis. Therefore, supplementation of L-cysteine can be useful when there is a risk of acrlamide toxicity.


Acrylamide, Testis, Oxidative stress, L-cysteine, Testosterone

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