Assessment of in-vivo anti-diabetic and anti-diarrheal effects of Flemingia stricta Roxb. leaf

  • Md. Shahrear Biozid Department of Pharmacy, International Islamic University Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
  • Mohammad Nazmul Alam Department of Pharmacy, International Islamic University Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
  • Md. Jainul Abeden Department of Pharmacy, International Islamic University Chittagong, Bangladesh
  • Md. Faruk Department of Pharmacy, International Islamic University Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
  • Ahmad Ibtehaz Chowdhury Department of Pharmacy, International Islamic University Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
  • Muzahidul Islam Sajib Department of Pharmacy, International Islamic University Chittagong, Bangladesh
  • Md. Masudur Rahman Department of Pharmacy, International Islamic University Chittagong, Bangladesh
  • Md. Rafikul Islam Department of Pharmacy, International Islamic University Chittagong, Bangladesh
Keywords: Flemingia stricta, Anti-diabetic, Anti-diarrheal, Alloxan, Metformin, Loperamide, Castor oil

Abstract

The objective of this study was to evaluate the anti-diabetic and anti-diarrheal activity of methanol extract of Flemingia stricta Roxb. (Fabaceae) leaf. In anti-diabetic study, the extract was administered to alloxan-induce diabetic mice at two concentrations (200 mg/kg and 400 mg/kg body weight) for acute (12 hours) and prolong treatments (15 days) and blood glucose levels of diabetic mice were monitored at intervals of hours and days throughout the duration of treatment. Antidiarrheal test was conducted by castor oil induced diarrhea and enteropooling as well as intestinal motility in mice at three different concentration (100 mg/kg, 200 mg/kg and 400 mg/kg body weight). Treatment of alloxan induce diabetic mice with the extract caused a significant reduction in fasting blood glucose level of the diabetic mice both in acute (12 hours) and prolong treatment (15 days) and it was determined that the F. stricta methanol extract at both concentration (200 mg/kg and 400 mg/kg) showed the significant (P<0.05) hypoglycemic effect in comparison to the standard drug metformin. In the case of castor oil induced diarrheal test, enteropooling test and gastrointestinal motility test, the extract of F. stricta  at 100 mg/kg, 200 mg/kg and 400 mg/kg has given significant effect (P<0.05) compared to the standard drug loperamide. But 400 mg/kg demonstrated the highest activity amongst the three doses. These results suggested that the methanol extract of F. stricta Roxb. possess promising anti-diabetic effect on alloxan-induced mice and significant antidiarrheal effect on castor oil induced diarrheal mice.

DOI: http://dx.doi.org/10.5281/zenodo.3839749

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References

1. Semwal DK, Badoni R, Semwal R, Kothiyal SK, Singh GJ, Rawat U. The genus Stephania (Menispermaceae): chemical and pharmacological perspectives. J Ethnopharmacol. 2010; 132: 369-383.

2. Tsay HS, Agrawal DC. Tissue culture technology of Chinese medicinal plant resources in Taiwan and their sustainable utilization. Int J App Sci Eng. 2005; 3: 215-223.

3. Robbers JE, Speedle MK, Tyler VE Tyler. Pharmacognosy and pharmacobiotechnology. Williams and Wilkins, Baltimore, USA; 1996.

4. Flemingia stricta Roxb., Fl. Ind. 3: 342. 1832; Hook. f., Fl. Brit. India 2: 228. 1876; Gamble, Fl. Pres. Madras 378(266). 1918; Sanjappa, Legumes Ind. 178. 1992; Mohanan & Sivad., Fl. Agasthyamala 211. 2002.

5. http://www.legumes-online.net/ildis/aweb/td076/td_16024.htm [Accessed on 2018 Aug 5].

6. Motaleb MA, Hossain MK, Alam MK, Mamun MA, Sultana M. Commonly used medicinal herbs and shrubs by traditional herbal practitioners Glimpses from Thanchiupazila of Bandarban, 2013: 88-89.

7. Rahman MA, Uddin SB, Wilcock CC. Medicinal plants used by Chakma Tribe in Hill Tracts Districts of Bangladesh. Indian J Tradit Knowl. 2007; 6(3): 508-517.

8. Uddin SN. Traditional uses of ethnomedicinal plants of the Chittagong Hill Tracts. Bangladesh National Herbarium. Mirpur 1, Dhaka 1216, Bangladesh, 2006.

9. Khisha T, Karim R, Chowdhury SR, Banoo R. Ethnomedical studies of Chakma communities of Chittagong Hill Tracts, Bangladesh. Bangl Pharmac J. 2012; 15(1): 59-67.

10. International Diabetes Federation. IDF diabetes atlas. 8th ed. Brussells: International Diabetes Federation; 2017.

11. King H, Aubert RE, Herman WH. Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections. Diabetes Care. 1998; 21: 1414-1431.

12. American Diabetes Association® Issues First Updates to 2018 Standards of Medical Care in Diabetes. http://www.diabetes.org/newsroom/press-releases/2018/standards-of-care-update-april-2018.html [Accessed on 19th August, 2018].

13. Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for 2000 and projections for 2030. Diabetes Care. 2004; 27: 1047-1053.

14. Rahman MM, Islam MS, Ali MS, Islam MR, Hossain MZ. Antidiabetic and cytotoxic activities of methanolic extract of Tabernaemontana divaricata (L.) flowers. Int J Drug Dev Res. 2011; 3: 270-276.

15. GBD 2015 Mortality and Causes of Death Collaborators. Global, regional, and national life expectancy, all-cause and cause-specific mortality for 249 causes of death, 1980-2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet. 2016; 388: 1459-1544.

16. Bustreo F, Okwo-Bele JM, Kamara L. World Health Organization perspectives on the contribution of the Global Alliance for Vaccines and Immunization on reducing child mortality. Arch Dis Child. 2015; 100(Suppl. 1): S34-S37.

17. Diarrhoeal disease: UNICEF DATA. June 2018. https://data.unicef.org/topic/child-health/diarrhoeal-disease/ [Accessed on: 19th August, 2018].

18. Mokomane M, Kasvosve I, de Melo E, Pernica JM, Goldfarb DM. The global problem of childhood diarrhoeal diseases: emerging strategies in prevention and management. Ther Adv Infect Dis. 2018; 5(1): 29-43.

19. Miller LC, Tainter ML. Estimation of ED50 or LD50 values and their error using logarithmic-probit graph paper. Proc Soc Med. 1944; 57: 261-264.

20. Antia BS, Okokon JE, Umoh EE, Udobang JA. Antidiabetic activity of ethanolic leaf extract of Panicum maximum. Int J Drug DevRes. 2010; 2(3): 488-492.

21. Shoba FG, Thomas M. Study of antidiarrhoeal activity of four medicinal plants in castor-oil induced diarrhoea. J Ethnopharmacol. 2001; 76(1): 73-76.

22. Uddin SJ, Sjilpi JA, Alam SM, Alamgir M, Rahman MT, SarkerSD. Antidiarrhoeal activity of the methanol extract of the barks of Xylocarpus moluccensis in castor oil- and magnesium sulphate induced diarrhoea models in mice. J Ethnopharmacol. 2005; 101: 139-143.

23. Robert A, Nezamis JE, Lancaster C, Hanchar AJ, Klepper MS. Enteropooling assay, a test for diarrhea produced by prostaglandins. Prostagladins. 1976; 11: 809-828.

24. Qnais EY, Elokda AS, Ghalyun YA, Abdulla FA. Antidiarrhea activity of the aqueous extract of Punica granatum (Pomegranate) peels. Pharmaceut Biol. 2007; 45: 715-720.

25. Meite S, Nguessan JD, Bahi C, Yapi HF, Djaman AJ, Guede GF. Antidiarrhoeal activity of the ethyl acetate extract of Morinda morindoides in rats. Trop J Pharm Res. 2009; 8(3): 201-207.

26. Marona HRN, Lucchesi MBB. Protocol to refine intestinal motility test in mice. Lab Anim. 2004; 38: 257-260.

27. Bnouham M, Ziyyat A, Mekhfi H, Tahri A, Legssyer A. Medicinal plants with potential antidiabetic activity-a review of ten years of herbal medicine research (1990-2000). Int J Diabetes Metab. 2006; 14: 1-25.

28. Kumar D, Kumar S, Kohli S, Arya R, Gupta J. Antidiabetic activity of methanolic bark extract of Albizia odoratissima Benth. in alloxan induced diabetic albino mice. Asian Pac J Trop Med. 2011; 4(11): 900-903.

29. Kumar R, Kumar PD, Prasad SK, Sairam K, Hemalatha S. Antidiabetic activity of alcoholic leaves extract of Alangium lamarckii Thwaites on streptozotocin-nicotinamide induced type 2 diabetic rats. Asian Pac J Trop Med. 2011; 4(11): 904-909.

30. Qamar F, Afroz S, Feroz Z, Siddiqui S, Ara A. Evaluation of hypoglycemic effect of Eassia italica. J Basic Appl Sci. 2011; 7(1): 61-64.

31. Saalmüller L. Ueberdiefetten Säuren des Ricinusöls [On the fatty acids of castor oil] [in German]. Justus Liebigs Ann Chem. 1848; 64: 108-126.

32. Mckeon TA, Lin JJ, Stafford AE. Biosynthesis of ricinoleate in castor oil. Adv Exp Med Biol. 1999; 464: 37-47.

33. Meyer H. Ueber den wirksamen Bestandtheil des Ricinusöls [On the active component of castor oil] [in German]. Arch Exp Path Pharmak. 1890; 28: 145-152.

34. Watson WC, Gordon RS. Studies on the digestion, absorption and metabolism of castor oil. Biochem Pharmacol. 1962; 11: 229-236.

35. Palombo EA. Phytochemicals from traditional medicinal plants used in the treatment of diarrhoea: Modes of action and effects on intestinal function. Phytother Res. 2006; 20: 717-724.

36. Yoshio K, Kazuko S, Bunsyo M, Kazunori H, Atsushi I, Yasuhiro K. Relationship between antidiarrhoeal effects of Hange-Shashin-To and its active components. Phytother Res. 1999; 13: 468-473.

37. Sorin T, Till FA, Rolf MN, Martin D, Stefan O. Castor oil induces laxation and uterus contraction via ricinoleic acid activating prostaglandin EP3 receptors. Proc Natl Acad Sci USA. 2012; 109(23): 9179-9184.

38. Romański, K. Effects of cholecystokinin-octapeptide and cerulein on ovine digestive motility under cholinergic blockade. Eur J Biol Res. 2017; 7: 31-49.

39. Akuodor GC, Muazzam I, Usman MI, Megwas UA, Akpan JL, Chilaka KC, et al. Evaluation of the antidiarrheal activity of methanol leaf extract of Bombax buonopozense in rats. Ibnosina J Med BS. 2011; 3(1): 15-20.
Published
2020-05-22
How to Cite
(1)
Biozid, M.; Alam, M.; Abeden, M.; Faruk, M.; Chowdhury, A.; Sajib, M.; Rahman, M.; Islam, M. Assessment of In-Vivo Anti-Diabetic and Anti-Diarrheal Effects of Flemingia Stricta Roxb. Leaf. European Journal of Biological Research 2020, 10, 156-166.
Section
Research Articles