Peppermint (Mentha piperita L.) essential oil as a potent anti-inflammatory, wound healing and anti-nociceptive drug
Keywords:
Topical anti-inflammatory, Peppermint essential oil, Menthol, Anti-nociceptive activity, Wound healing
Abstract
The present investigation was designed to study the chemical composition of Algerian peppermint essential oil (PEO) as well as the in vitro and in vivo anti-inflammatory, wound-healing and anti-nociceptive properties. Twenty-three compounds were identified in the PEO with the main chemical component as menthol (53.29%). Also, PEO showed a high content of oxygenated monoterpene compounds (92.75%). Topical application of PEO at doses of 200 and 20 µL/kg significantly reduced the acute ear edema in 38.09% and 36.50, respectively. Histological observation confirmed that PEO inhibited the skin inflammatory response. In-vivo wound healing activity of the cream prepared from PEO (0.5% w/w) was assessed by circular excision wound model followed by histological examination. The topical administration of PEO cream showed a significant decrease of unhealed wound area rate between the 6th (1.67±0.14 mm2) and the 9th (0.49±0.22 mm2) days of treatment when compared with the vehicle (2.32±0.77 mm2; p<0.05) and Madecassol® 0.1% creams (2.23±0.35 mm2; p<0.05). The PEO reduced nociceptive behavior at all doses tested in the acetic acid-induced nociception test (p<0.05). These findings support the anti-inflammatory, wound-healing and analgesic properties of PEO. We suggest that PEO is a promising candidate for use in skin care products with anti-inflammatory and wound-healing properties.
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References
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30. Atta AH, Alkofahi A. Anti-nociceptive and anti-inflammatory effects of some Jordanian medicinal plant extracts. J Ethnopharmacol. 1998; 60: 117-124.
31. Silva JM, Pereira CV, Mano F, Silva E, Castro VI, Sá-Nogueira I, Duarte ARC. Therapeutic role of deep eutectic solvents based on menthol and saturated fatty acids on wound healing. ACS Appl Bio Mater. 2019; 2: 4346-4355.
32. Liapi C, Anifantis G, Chinou I, Kourounakis AP, Theodosopoulos S, Galanopoulou P. Antinociceptive properties of 1, 8-cineole and β-pinene, from the essential oil of Eucalyptus camaldulensis leaves, in rodents. Plant Med. 2007; 73: 1247-1254.
33. Lima TC, Da Nóbrega FR, De Brito AEM, De Sousa DP. Analgesic-like activity of essential oil constituents: An update. Int J Mol Sci. 2017; 18: 2392.
2. Bauer SM, Bauer RJ, Velazquez OC. Angiogenesis, vasculogenesis, and induction of healing in chronic wounds. Vasc Endovasc Surg. 2005; 39: 293-306.
3. Sevinsky RE, Stewart DW, Harirforoosh S. Nonsteroidal anti-inflammatory drugs: Is there a link between cardiovascular and renal adverse effects? J Integr Nephrol Androl. 2017; 4: 1.
4. Süntar I, Tumen I, Ustün O, Keleş H, Akkol EK. Appraisal on the wound healing and anti-inflammatory activities of the essential oils obtained from the cones and needles of Pinus species by in vivo and in vitro experimental models. J Ethnopharmacol. 2012; 139: 533-540.
5. Tumen I, Süntar I, Keleş H, Küpeli Akkol E. A therapeutic approach for wound healing by using essential oils of Cupressus and Juniperus species growing in Turkey. Evid Based Complement Alternat Med. 2012; 2012: 728281.
6. Ibrahim NI, Wong SK, Mohamed IN, Mohamed N, Chin KY, Ima-Nirwana S, Shuid AN. Wound healing properties of selected natural products. Int J Environ Res Publ Health. 2018; 15: 2360.
7. Galeotti N, Mannelli LDC, Mazzanti G, Bartolini A, Ghelardini C. Menthol: a natural analgesic compound. Neurosci Lett. 2002; 322: 145-148.
8. Tumen I, Akkol EK, Suntar I, Erbey G, Kurtca M, Keles H, Pranovich A. Evaluation of the wound healing and anti-inflammatory activities and phytochemical analysis of Myrtus communis L. Fresenius Environ Bull. 2017; 26: 4420-4428.
9. Ogunwande IA, Avoseh ON, Igile DO, Lawal OA, Ascrizzi R, Guido F. Chemical Constituents, anti-nociceptive and anti-inflammatory activities of essential oil of Phyllanthus muellerianus. Natl Prod Comm. 2019; 14: 1934578X19846356.
10. Vakilian K, Atarha M, Bekhradi R, Chaman R. Healing advantages of lavender essential oil during episiotomy recovery: a clinical trial. Complement Therap Clin Pract. 2011; 17: 50-53.
11. McKay DL, Blumberg JB. A review of the bioactivity and potential health benefits of peppermint tea (Mentha piperita L.). Phytother Res. 2006; 20: 619-633.
12. Balakrishnan A. Therapeutic uses of peppermint-a review. J Pharm Sci Res. 2015; 7: 474.
13. Sun Z, Wang H, Wang J, Zhou L, Yang P. Chemical composition and anti-inflammatory, cytotoxic and antioxidant activities of essential oil from leaves of Mentha piperita grown in China. PloS One. 2014; 9(12): e114767.
14. Loolaie M, Moasefi N, Rasouli H, Adibi H. Peppermint and its functionality: A review. Arch Clin Microbiol. 2017; 8: 54.
15. Stanojevic LP, Stanojevic JS, Savic VL, Cvetkovic DJ, Kolarevic A, Marjanovic-Balaban Z, Nikolic LB. Peppermint and basil essential oils: chemical composition, in vitro antioxidant activity and in vivo estimation of skin irritation. J Essent Oil Bear Plants. 2019; 22: 979-993.
16. De Groot A, Schmidt E. Essential oils, part V: peppermint oil, lavender oil, and lemongrass oil. Dermatitis. 2016; 27: 325-332.
17. Neeraj T, Prakash A, Seema Y. Antimicrobial activity and medicinal values of essential oil of Mentha piperita L. Int J Eng Innov Technol. 2013; 2: 214-218.
18. Da Silva Ramos R, Rodrigues ABL, Farias ALF, Simões RC, Pinheiro MT, Ferreira RMDA, de Almeida SSMD. Chemical composition and in vitro antioxidant, cytotoxic, antimicrobial, and larvicidal activities of the essential oil of Mentha piperita L. (Lamiaceae). Scient World J. 2017; 2017: 4927214.
19. Anosike CA, Obidoa O, Ezeanyika LU. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU J Pharm Sci. 2012; 20: 76.
20. Niu X, Xing W, Li W, Fan T, Hu H, Li Y. Isofraxidin exhibited anti-inflammatory effects in vivo and inhibited TNF-α production in LPS-induced mouse peritoneal macrophages in vitro via the MAPK pathway. Int Immunopharmacol. 2012; 14: 164-171.
21. Boukhatem MN, Ferhat MA, Kameli A, Saidi F, Kebir HT. Lemon grass (Cymbopogon citratus) essential oil as a potent anti-inflammatory and antifungal drugs. Libyan J Med. 2014; 9: 25431.
22. Esetlili BÇ, Çobanoğlu Ö, Tepecik M, Öztürk B, Anaç D. Yield, essential nutrients and essential oils of peppermint (Mentha x piperita L.) grown under organic farming conditions. J Agr Fac Uludag Univ. 2015; 29: 29-36.
23. Mahboubi M, Kazempour N. Chemical composition and antimicrobial activity of peppermint (Mentha piperita L.) essential oil. Songkl J Sci Technol. 2014; 36: 83-87.
24. Yang SA, Jeon SK, Lee EJ, Shim CH, Lee IS. Comparative study of the chemical composition and antioxidant activity of six essential oils and their components. Nat Prod Res. 2010; 24: 140-151.
25. Abdel-Hameed ESS, Salman MS, Fadl MA, Elkhateeb A, El-Awady MA. Chemical composition of hydrodistillation and solvent free microwave extraction of essential oils from Mentha piperita L. growing in Taif, Kingdom of Saudi Arabia, and their anticancer and antimicrobial activity. Orient J Chem. 2018; 34: 222-233.
26. Parvin MS, Das N, Jahan N, Akhter MA, Nahar L, Islam ME. Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark. BMC Res Notes. 2015; 8: 412.
27. Mogosan C, Vostinaru O, Oprean R, Heghes C, Filip L, Balica G, Moldovan RI. A comparative analysis of the chemical composition, anti-inflammatory, and antinociceptive effects of the essential oils from three species of Mentha cultivated in Romania. Molecules. 2017; 22: 263.
28. Andrade LN, De Sousa DP. A review on anti-inflammatory activity of monoterpenes. Molecules. 2013; 18: 1227-1254.
29. Zaia MG, Cagnazzo TDO, Feitosa KA, Soares EG, Faccioli LH, Allegretti SM, Anibal FDF. Anti-inflammatory properties of menthol and menthone in Schistosoma mansoni infection. Front Pharmacol. 2016; 7: 170.
30. Atta AH, Alkofahi A. Anti-nociceptive and anti-inflammatory effects of some Jordanian medicinal plant extracts. J Ethnopharmacol. 1998; 60: 117-124.
31. Silva JM, Pereira CV, Mano F, Silva E, Castro VI, Sá-Nogueira I, Duarte ARC. Therapeutic role of deep eutectic solvents based on menthol and saturated fatty acids on wound healing. ACS Appl Bio Mater. 2019; 2: 4346-4355.
32. Liapi C, Anifantis G, Chinou I, Kourounakis AP, Theodosopoulos S, Galanopoulou P. Antinociceptive properties of 1, 8-cineole and β-pinene, from the essential oil of Eucalyptus camaldulensis leaves, in rodents. Plant Med. 2007; 73: 1247-1254.
33. Lima TC, Da Nóbrega FR, De Brito AEM, De Sousa DP. Analgesic-like activity of essential oil constituents: An update. Int J Mol Sci. 2017; 18: 2392.
Published
2020-05-16
How to Cite
(1)
Kehili, S.; Boukhatem, M.; Belkadi, A.; Ferhat, M.; Setzer, W. Peppermint (Mentha Piperita L.) Essential Oil As a Potent Anti-Inflammatory, Wound Healing and Anti-Nociceptive Drug. European Journal of Biological Research 2020, 10, 132-149.
Section
Research Articles
