Ameliorating effect of quercetin against UV radiation-induced damage in Drosophila melanogaster

  • Susmita Majumder Post Graduate Department of Zoology, Asutosh College, Kolkata, West Bengal, India
  • Mohna Bandyopadhyay Department of Dermatology, University of Pittsburgh, Pennsylvania, USA
  • Sandip Pal Department of Zoology, Barrackpore Rastraguru Surendranath College, Barrackpore, West Bengal, India
  • Dalia Mukhopadhyay Post Graduate Department of Zoology, Asutosh College, Kolkata, West Bengal, India
Keywords: Quercetin, Flavonoid, UV radiation, Radioprotection, Fruit fly, Eclosion

Abstract

Quercetin is a plant flavonoid found in various fruits, leaves such as tea, vegetables and has been extensively studied due to its antioxidative, anticancer, anti-inflammatory and anti-neurodegenarative effects. UV radiation is harmful for human being as it may cause several complications such as skin cancer. Fruit fly (Drosophila sp.) has long been used as an arthropod model for genetics related studies. In the present study, the protective effect of quercetin is evaluated against UV-C radiation induced damage using Drosophila melanogaster. Pre-treatment with quercetin (10 µM) recovered the shortened lifespan caused by UV radiation and has also increased eclosion rate and the dose of quercetin is lower than the previously reported doses of other flavonoids. Flies subjected to moderate dose of UV radiation showed distinct abnormal characters such as incomplete abdominal pigmentation, curly wings or outstretched wings, whereas quercetin pretreatment showed no such abnormal characters or mutant phenotypes.  There is a considerable amount of change in the eclosed adult fly size, pupal size and pupal migration distance as well. Gel electrophoresis study of salivary gland DNA of D. melanogaster demonstrates the efficacy of quercetin in conferring protection to DNA against UV radiation-induced damage. Therefore, it can be concluded that quercetin may act as an effective protective agent against UV radiation-induced damage.

DOI: http://dx.doi.org/10.5281/zenodo.3588543

References

1. Pal S, Saha C. A review on structure-affinity relationship of dietary flavonoids with serum albumins. J Biomol Struct Dyn. 2014; 32: 1132-1167.

2. Jan AT, Kamli MR, Murtaza I, Singh JB, Ali A, Haq QMR. Dietary flavonoid quercetin and associated health benefits - an overview. Food Rev Int. 2010; 26(3): 302-317.

3. Liu RH. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr. 2003; 78(3): 517-520.

4. Pietta PG. Flavonoids as antioxidants. J Nat Prod. 2000; 63: 1035-1042.

5. Dajas F. Life or death: Neuroprotective and anticancer effects of quercetin. J Ethnopharmacol. 2012; 143(2): 383-396.

6. Guardia T, Rotelli AE, Juarez AO, Pelzer LE. Anti-inflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. II Farmacol. 2001; 56(9): 683-687.

7. Pal S, Saha C, Dey SK. Studies on black tea (Camellia sinensis) extract as a potential antioxidant and a probable radioprotector. Radiat Environ Biophys. 2013; 52(2): 269-278.

8. Benkovic V, Knezevic AH, Dikic D, Lisicic D, Orsolic N, Basic I. Radioprotective effects of propolis and quercetin in
Published
2019-12-20
How to Cite
Majumder, S., Bandyopadhyay, M., Pal, S., & Mukhopadhyay, D. (2019). Ameliorating effect of quercetin against UV radiation-induced damage in Drosophila melanogaster. European Journal of Biological Research, 9(4), 276-285. Retrieved from http://journals.tmkarpinski.com/index.php/ejbr/article/view/234
Section
Research Articles