In vitro evaluation of antimicrobial activities from aqueous and methanolic extracts of cyanobacteria

  • Moein Safari Department of Biology, Faculty of Basic Science, Ilam University, Ilam, Iran
  • Salman Ahmady-Asbchin Department of Molecular and Cell Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran
  • Pantea Zamanifar Department of Biology, Faculty of Basic Science, Islamic Azad University, Varamin-Pishva branch, Tehran, Iran
Keywords: Cyanobacteria, Antimicrobial activity, Disc diffusion, Broth microdilution, Staphylococcus aureus


In this present study, antimicrobial activities of aqueous and methanolic extracts of cyanobacteria against some of fungi and pathogenic bacteria were investigated. Cyanobacteria strains Fischerella ambigua ISC67 and Schizothrix vaginata ISC108 were cultured in BG-11 medium. Extraction was performed by adding the solvent to cyanobacterial biomass and then filtering and drying of the mixture. The antimicrobial activity was evaluated by disc diffusion method and broth microdilution method was applied to determine the minimum inhibitory concentration. The results show that the aqueous and methanolic extracts of F. ambigua has a significant antimicrobial effect while, the tested extracts of S. vaginata was no significant antibacterial and antifungal activity. Highest antibacterial activity from aqueous extract of F. ambigua was against S. aureus (PTCC 1112) which the average zone diameter around it was 33.33 mm. The antibacterial effect of aqueous extracts against Gram-positive bacteria was more than Gram-negative bacteria significantly. Antifungal activity showed that methanolic extract of F. ambigua have significant antifungal activity. Minimum inhibitory concentration of active extract against most tested bacterial and fungal was 125 mg/ml. The present study has proved that the aqueous and methanolic extracts of F. ambigua possessed strong antibacterial and antifungal properties against the pathogenic microorganism. Therefore, cyanobacteria can be a rich source for natural products with antimicrobial activity.



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1. Mercy R, David Udo E. Natural products as lead bases for drug discovery and development. Res Rep Med Sci. 2018; 2(1): 1-2.

2. Swain SS, Paidesetty SK, Padhy RN. Antibacterial, antifungal and antimycobacterial compounds from cyanobacteria. Biomed Pharmacother. 2017; 90: 760-776.

3. Abazari M, Zarrini G, Rasooli I. Antimicrobial potentials of Leptolyngbya sp. and its synergistic effects with antibiotics. Jundishapur J Microbiol. 2013; 6(5): 1-6.

4. Paerl HW, Hall NS, Calandrino ES. Controlling harmful cyanobacterial blooms in a world experiencing anthropogenic and climatic-induced change. Sci Total Environ. 2011; 409: 1739-1745.

5. Soltani N, Khavari-Nejad RA, Yazdi MT, Shokravi S, Fernández-Valiente E. Screening of soil cyanobacteria for antifungal and antibacterial activity. Pharm Biol. 2005; 43: 455-459.

6. Kumar M, Kumar MT, Srivastava A, Kumar G.J, Kumar SR, Tilak R, Asthana RK. Cyanobacteria, Lyngbya aestuarii and Aphanothece bullosa as antifungal and antileishmanial drug resources. Asian Pac J Trop Biomed. 2013; 3(6): 458-463.

7. Sakthivel K, Kathiresan K. Antimicrobial activities of marine cyanobacteria isolated from mangrove environment of south east coast of India. J Nat Prod. 2012; 5: 147-156.

8. Rastogi RP, Sinha RP. Biotechnological and industrial significance of cyanobacterial secondary metabolites. Biotechnol Adv. 2009; 27: 521-539.

9. Mundt S, Kreitlow S, Nowotny A, Effmert U. Biological and pharmacological investigation of selected cyanobacteria. Int J Hyg Environ Health. 2001; 203: 327-334.

10. Moharram A, Zohri A, Omar H, Abd El-Ghani O. In vitro assessment of antimicrobial and anti-inflammatory potential of endophytic fungal metabolites extracts. Eur J Biol Res. 2017; 7(3): 234-244.

11. Volk R, Furkert FH. Antialgal, antibacterial and antifungal activity of two metabolites produced and excreted by cyanobacteria during growth. Microbiol Res. 2006; 161: 180-186.

12. Najdenski HM, Gigova LG, Iliev II, Pilarski PS, Lukavsky J, Tsvetkova IV, Ninova MS, Kussovski VK. Antibacterial and antifungal activities of selected microalgae and cyanobacteria. Int J Food Sci Technol. 2013; 48: 1533-1540.

13. Chandra K, Rajashekhar M. Antimicrobial activity of freshwater cyanobacteria isolated from pharmaceutical wastes. Afr J Microbiol Res. 2013; 7(17): 1757-1765.

14. Val AG, Platas G, Basilio A, Cabello A, Gorrochategui J, Suay I, et al. Screening of antimicrobial activities in red, green and brown macroalgae from Gran Canaria (Canary Islands, Spain). Int Microbiol. 2001; 4(1): 35-40.

15. Ahmady-Asbchin S, Safari M, Moradi H, Sayadi V. Antibacterial effects of methanolic and ethanolic leaf extract of Medlar (Mespilus germanica) against bacteria isolated from hospital environment. Arak Med Univ J. 2013; 16(75): 1-13.

16. Nain P, Kumar A, Sharma S, Nain J. In vitro evaluation of antimicrobial and antioxidant activities of methanolic extract of Jasminum humile leaves. Asian Pac J Trop Med. 2011; 4(10): 804-807.

17. Clinical and Laboratory Standards Institute. In: Performance Standards for Antimicrobial Susceptibility Testing. 27th M100. Clinical and Laboratory Standards Institute, PA, USA, 2017.

18. Bączek KB, Kosakowska O, Przybyl J, Pióro-Jabrucka E, Costa R, Mondello L, et al. Antibacterial and antioxidant activity of essential oils and extracts from costmary (Tanacetum balsamita L.) and tansy (Tanacetum vulgare L.). Indust Crops Prod. 2017; 102: 154-163.

19. Safari M, Ahmady-Asbchin S, Soltani N. In vitro assessment of antimicrobial activity from aqueous and methanolic extracts of some species of cyanobacteria. Biol J Microorganism. 2015; 4(14): 111-130.

20. Priyadharshini R, Ambikapathy V, Pavai T. In vitro antimicrobial activity of Oscillatoria angustissima. Int J Adv Res. 2013; 1(4): 60-68.

21. Mitani T, Ota K, Inaba N, Kishida K, Koyama HA. Antimicrobial activity of the phenolic compounds of Prunus mume against Enterobacteria. Biol Pharm Bull. 2018; 41: 208-212.

22. Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Curr Med Chem. 2015; 22: 132-149.

23. Madhumathi V, Deepa P, Jeyachandram S. Antimicrobial activity of cyanobacteria isolated from freshwater lake. Int J Microbiol Res. 2011; 2(3): 213-216.

24. Chauhan A, Chauhan G, Gupta P.C, Goyal P, Kaushik P. In vitro antibacterial evaluation of Anabaena sp. against several clinically significant microflora and HPTLC analysis of its active crude extracts. Indian J Pharmacol. 2011; 42(2); 105-107.

25. Shan L, He P, Sheen J. Intercepting host MAPK signaling cascades by bacterial type III effectors. Cell Host Microbe. 2007; 1: 167-174.

26. Rath B, Priyadarshani I. Antibacterial and antifungal activity of marine cyanobacteria from Odisha Coast. Int J Curr Res. 2013; 2(1): 248-251.

27. Ghasemi Y, Tabatabaei-Yazdi M, Shokravi S, Soltani N, Zarrini G. Antifungal and ‎antibacterial activity of paddy-fields cyanobacteria from the north of Iran. J ‎Sci Islamic Rep Iran. 2003; 14(3): 203-209‎.

28. Smitka J, Boyer L, Zimba PV. Impacts of noxious and odorous cyanobacterial metabolites on aquaculture systems. Aquaculture. 2008; 280: 5-20.

29. Challouf R, Trabelsi L, Dhieb RB, Abed OE, Yahia A, Ghozzi K, et al. Evaluation of cytotoxicity and biological activities in extracellular polysaccharides released by cyanobacterium Arthrospira platensis. Braz Arch Biol Technol. 2011; 54: 831-838.
How to Cite
Safari, M.; Ahmady-Asbchin, S.; Zamanifar, P. In Vitro Evaluation of Antimicrobial Activities from Aqueous and Methanolic Extracts of Cyanobacteria. European Journal of Biological Research 2019, 9, 184-192.
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