Antifungal activity of the rhizome extracts of Pulsatilla vulgaris against Candida glabrata
Keywords:
Pulsatilla vulgaris subsp. vulgaris, Ranunculaceae, Leaves and rhizomes extracts, Biological activity, Microbiological assays
Abstract
Pulsatilla vulgaris Mill. (“Pasque flower”, Ranunculaceae) is rare and a threatened plant species in Europe. It produces biologically active secondary metabolites. P. vulgaris is also known herbal drug used for centuries in traditional Chinese and Korean medicine. The rhizomes of P. vulgaris have been traditionally used for treatment of headaches, neuralgia, insomnia, hyperactivity, bacterial skin infections, septicemia, cough and bronchitis. In the present study, the extracts of leaves and rhizomes of P. vulgaris were evaluated for their antifungal, antimicrobial, antimalarial and cytotoxic activities. The results showed the antifungal activity of crude extracts of the rhizome of P. vulgaris against the yeast Candida glabrata with an IC50 of 11 µg/ml. These results indicate that the selected medicinal plant could be further investigated for identifying compounds that may be responsible for the observed activity and that may represent new leads in fungal drug discovery.
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References
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3. Cheon SA, Choi BK, Jeong CS, Li DW, Lee EB. The anti-inflammatory and analgesic actions of the root of Pulsatilla koreana. Korean J Pharmacogn. 2000; 31: 174-184.
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6. Kim Y, Bang SC, Lee JH, Ahn BZ. Pulsatilla saponin D: the antitumor principle from Pulsatilla koreana. Arch Pharm Res. 2004; 27: 915-918.
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17. Xu HJ, Shi XW, Ji X, Du YF, Zhu H, Zhang LT. A rapid method for simultaneous determination of triterpenoid saponins in Pulsatilla turczaninovii using microwave-assisted extraction and high performance liquid chromatography-tandem mass spectrometry. Food Chem. 2012; 135: 251-258.
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19. Rolski S, Przyborowski L. Dissert Pharm. 1961; 13: 349-355.
20. Ye WC, Ji NN, Zhao SX, Che CT. A New Cytotoxic Saponin from Pulsatilla patens var. multifida. Pharm Biol. 2001; 39: 7-10.
21. Danova K, Bertoli A, Pistelli L, Dimitrow D, Pistelli L. In vitro culture of Balkan endemic and rare Pulsatilla species for conservational purposes and secondary metabolites production. Bot Serb. 2009; 33: 157-162.
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23. Łaska G, Sienkiewicz A, Piotrowska-Niczyporuk A. New Polish species with isolated Pulsatilla saponin D. Planta Med. 2016; 82: S1-S381.
24. Luft G. The distribution of saponins and tannins in the plant. Monatshefte fuer Chemie 1926; 47: 259-284.
25. Raymond-Hamet L. Pharmacodynamic properties of Anemone pulsatilla. Bull Sci Pharmacol. 1927; 34: 143-151.
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28. Liu DD, Chao WM, Turgeon R. Transport of sucrose, not hexose, in the phloem. J Exp Bot. 2012; 63: 4315-4320.
29. Hensen I, Oberprieler C, Wesche K. Genetic structure, population size, and seed production of Pulsatilla vulgaris Mill. (Ranunculaceae) in Central Germany. Flora 2005; 200: 3-14.
30. DiLeo MF, Graf R, Holderegger R, Rico Y, Wagner HH. Highly polymorphic microsatellite markers in Pulsatilla vulgaris (Ranunculaceae) using next-generation sequencing. Appl Plant Sci. 2015; 3(7): apps.1500031.
31. Walker KJ, Pinches CE. Reduced grazing and the decline of Pulsatilla vulgaris Mill. (Ranunculaceae) in England. UK. Biol Conserv. 2011; 144: 3098-3105.
32. Piqueray J, Saad L, Bizoux JP, Mahy G. Why some species cannot colonise restored habitats? The effects of seed and microsite availability. J Nat Conserv. 2013; 21: 189-197.
33. Pfeifer E, Holderegger R, Matthies D, Rutishauser R. Investigation on the population biology of a flagship species of dry meadows: Pulsatilla vulgaris Mill. in north-eastern Switzerland. Bot Helv. 2002; 112: 153-172.
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43. Foster S, Johnson R. National geographic desk reference to natural medicine. National Geographic, Washington, 2006.
44. Baer H, Holden M, Seegal BC. The nature of the antibacterial agent from Anemone pulsatilla. J Biol Chem. 1946; 162: 65-68.
45. Duan H, Zhang Y, Xu J, Qiao J, Suo Z, Hu G, et al. Effect of anemonin on NO, ET-1 and ICAM-1 production in rat intestinal microvascular endothelial cells. J Ethnopharmacol. 2006; 104: 362-366.
46. Bang SC, Lee JH, Song G, Kim DH, Yoon MT, Ahn BZ. Antitumor activity of Pulsatilla koreana saponins and their structure-activity relationship. Chem Pharm Bull. 2005; 53: 1451-1454.
47. NCCLS: National Committee on Clinical Laboratory Standards, 2002; 22: 15.
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52. Makler MT, Hinrichs DJ. Measurement of the lactate dehydrogenase activity of Plasmodium falciparum as an assessment of parasitemia. Am J Trop Med Hyg. 1993; 48: 205-210.
53. Borenfreund E, Babich H, Martin-Alguacil N. Rapid chemosensitivity assay with human normal and tumor cells in vitro. In Vitro Cell Dev Biol. 1990; 26: 1030-1034.
54. Xu QM, Shu Z, He WJ, Chen LY, Yang SL, Yang G, et al. Antitumor activity of Pulsatilla chinensis (Bunge) Regel saponins in human liver tumor 7402 cells in vitro and in vivo. Phytomedicine 2012; 19: 293-300.
55. Hong SW, Jung KH, Lee H, Son MK, Yan HH, Kang NS, et al. SB365, Pulsatilla saponin D, targets c-Met and exerts antiangiogenic and antitumor activities. Carcinogenesis 2013; 34: 2156-2169.
56. Han CK, Park YH, Jin DQ, Hwang YK, Oh KB, Hand JS. SK-PC-B70M from Pulsatilla koreana improves scopolamine-induced impairments of memory consolidation and spatial working memory. Brain Res. 2007; 1184: 254-259.
57. Seo JS, Kim TK, Leem YH, Lee KW, Park SK, Baek IS, et al. SK-PC-B70M confers anti-oxidant activity and reduces β levels in the brain of Tg2576 mice. Brain Res. 2009; 1261: 100-108.
58. Yoo HH, Lee SK, Lim SY, Kim Y, Kang MJ, Kim EJ, et al. LC-MS/MS method for determination of hederacolchiside E, a neuroactive saponin from Pulsatilla koreana extract in rat plasma for pharmacokinetic study. J Pharm Biomed Anal. 2008; 48: 1425-1429.
59. Liu JY, Guan YL, Zou LB, Gong YX, Hua HM, Xu YN, et al. Saponins with neuroprotective effects from the roots of Pulsatilla cernua. Molecules 2012; 17: 5520-5531.
60. Dai L, Wang H, Chen Y. The immune enhancing effect of PcG2A-a glycoprotein isolated from dried root of Pulsatilla chinensis (Bunge) Regel. Chin J Biochem Pharm. 2000; 21: 230-231.
61. Li HB, Wong CC, Cheng KW, Chen F. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. Food Sci Tech. 2008; 41: 385-390.
62. Son MK, Jung KH, Lee HS, Lee H, Kim SJ, Yan HH, et al. SB365, Pulsatilla saponin D suppresses proliferation and induces apoptosis of pancreatic cancer cells. Oncol Rep. 2013; 30: 801-808.
63. Fidel F, Vazquez J, Sobel J. Candida glabrata: review of epidemiology, pathogenesis, and clinical disease with comparison to C. albicans. Clin Microbiol Rev. 1999; 12: 80-96.
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69. Butaye J, Adriaens D, Honnay O. Conservation and restoration of calcareous grasslands: A concise review of the effects of fragmentation and management on plant species. Biotechnol Agro Soc Environ. 2005: 9: 111-118.
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Published
2019-05-25
How to Cite
(1)
Łaska, G.; Sienkiewicz, A. Antifungal Activity of the Rhizome Extracts of Pulsatilla Vulgaris Against Candida Glabrata. European Journal of Biological Research 2019, 9, 93-103.
Section
Research Articles
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