Effect of hormone for in vitro propagation of Asparagus racemosus Wild.

  • Niroj Paudel Department of Applied Plant Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
  • Mukti Ram Aryal Department of Botany, Tri-Chandra multiple Campus (Tribhuvan University), Nepal
  • Rudra Hari Puri Department of Botany, Tri-Chandra multiple Campus (Tribhuvan University), Nepal
Keywords: Asparagus racemosus Wild., 6-benzyl amino purine (BAP), Growth and development, In vitro propagation, Medicinal plant, 1-napthalene acetic acid (NAA)


Asparagus racemosus Wild. is an undershrub, climber which is extremely branched with woody stem and spines like reduced leaves. It founds in tropical and sub-tropical region of Nepal up to 1500 meter above sea level. A. racemosus Wild. is an endangered species widely used in modern drug development and Ayurveda as well. The subculture explant shows responses to the different concentration of hormone. The composition of MS + 0.5 mg/l BAP and MS + NAA 0.5 mg/l + kinetin 1 mg/l most significant for shoot multiplication. Over them, the composition MS = 0.01 mg/l NAA + 1.0 mg/l BAP along with MS + 0.5 mg/l NAA = 1.5 mg/l kinetin is effective for the development of shoot in Asparagus racemosus. The hormone play important role on the regeneration of shoot in vitro condition. The knowledge of hormonal requirement help to promote the development and growth of endangered plants for their rapid propagation.

DOI: http://dx.doi.org/10.5281/zenodo.1407185


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How to Cite
Paudel, N., Aryal, M., & Puri, R. (2018). Effect of hormone for in vitro propagation of Asparagus racemosus Wild. Current Life Sciences, 4(4), 53-61. Retrieved from http://journals.tmkarpinski.com/index.php/cls/article/view/81
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