Analysis of genetic diversity of certain species of Aristolochia using ISSR-based molecular markers

Bhaskar Sarma, Bhaben Tanti

Abstract


Inter Simple Sequence Repeat (ISSR) polymorphism was used to determine genetic diversity and phylogenetic relationship in 4 species of Aristolochia viz A. indica Linn., A. saccata Wall., A. tagala Cham. and A. cathcartii Hook.f & T. A panel of 8 ISSR primers was screened representing di-nucleotide repeats, of which 57 polymorphic and informative patterns were selected to determine the genetic diversity. The consensus tree constructed using binary data from banding patterns generated by ISSR-PCR depicting that A. indica and A. cathcartii are more similar in terms of their genetic level and included in the same cluster. However, the rest of the two species viz., A. saccata and A. tagala are included into two different clusters which indicate their level of polymorphisms in the genetic level. This study may be useful in identifying diverse genetic stocks of Aristolochia, which may then be conserved on a priority basis. The ISSR markers are thus useful in the fingerprinting of wild species germplasm, and in understanding the evolutionary relationships of Aristolochia.


Keywords


Aristolochia indica; Aristolochia saccata; Aristolochia tagala; Aristolochia cathcartii; ISSR; Genetic diversity

Full Text:

PDF

References


Che CT, Almed MS, Kang SS, Waller DP, Bengel AS, Martin A, et al. Studies on Aristolochia III. Isolation and biological evaluation of constituents of Aristolochia indica roots for fertility regulating activity. J Nat Prod. 1984; 47(2): 331-341.

Das R, Kausik A, Pal TK. Anti-inflammatory activity study of antidote Aristolochia indica to the venom of Heteropneustes fossilis in rats. J Chem Pharm Res. 2010; 2(2): 554-562.

Sarma B, Tanti B. Karyomorphology of three species of Aristolochia - rare and endemic medicinal plants of Assam, India. Caryologia Int J Cytol Cytosyst Cytogen. 2015; 68(2): 154-158.

Neinhuis C, Wanke S, Hilu KW, Müller K, Borsch T. Phylogeny of Aristolochiaceae based on parsimony, likelihood and Bayesian analyses of trnL-trnF sequences. Plant Syst Evol. 2005; 250(1-2): 7-26.

Wanke S, Gonzales F, Neinhuis C. Systematics of Pipevines: combining morphological and fast-evolving molecular characters to investigate the relationships within subfamily Aristolochioideae (Aristolochiaceae). Int J Plant Sci. 2006; 167(6): 1215-1227.

Wanke S, Jaramillo MA, Borsch T, Samain MS, Quandt D, Neinhuis C. Evolution of Piperales - matK gene and trnK intron sequence data reveal lineage specific resolution contrast. Mol Phylogen Evol. 2007; 42(2): 477-497.

Wu TS, Tsai YL, Damu AG, Kuo PC, Wu PL. Constituents from the root and stem of Aristolochia elegans. J Nat Prod. 2002; 65(11): 1522-1525.

Staub JE, Serquen FC, Gupta M. Genetic markers, map construction, and their application in plant breeding. Hort Sci. 1996; 31(5): 729-739.

Gupta PK, Varshney RK. The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica. 2000; 113(3): 163-185.

Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR) - anchored polymerase chain reaction amplification. Genomics. 1994; 20(2): 176-183.

Gupta M, Chyi YS, Romero-Severson J, Owen JL. Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats. Theor Appl Genet. 1994; 89(7-8): 998-1006.

Wu K, Jones R, Dannaeberger L, Scolnik PA. Detection of microsatellite polymorphisms without cloning. Nucleic Acids Res. 1994; 22(15): 3257-3258.

Meyer W, Mitchell TG, Freedman EZ, Vilgays R. Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans. J Clin Microbiol. 1993; 31(9): 2274-2280.

Godwin ID, Aitken EAB, Smith LW. Application of inter-simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis. 1997; 18(9): 1524-1528.

Tautz D, Renz M. Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Res. 1984; 12(10): 4127-4138.

Orhan E, Ercisli S, Yildirim N, Agar G. Genetic variation among mulberry genotypes (Morus alba) as revealed by random amplified polymorphic DNA (RAPD) markers. Plant Syst Evol. 2007; 265(3-4): 251-258.

Rahman A, Tanti B, Sarma GC, Kalita J. Genetic diversity of Persea bombycina from Goalpara district of Assam, India. Adv Biosci Biotech. 2012; 3(1): 20-24.

Sambrook J, Fritsch E, Maniatis T. Molecular cloning: a laboratory manual, vol. I. 2nd edn. Cold Spring Harbor Laboratory Press, 1989.

Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990; 18(22): 6531-6535.

Roldan-Ruiz I, Dendauw J, Van Bockstaele E, Depicker A, Loose MD. AFLP markers reveal high polymorphic rates in rye grasses (Lolium spp.). Mol Breed. 2000; 6(2): 125-134.

Jaccard P. Nouvelles researches Sur la distribution florale. Bull Soc Vaud Sci Nat. 1908; 44: 223-270.

Rohlf FJ. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.2. Exeter Software, Setauket, NY, 2000.

Reddy MP, Sarla N, Neeraja CN, Siddiq EA. Assessing genetic variation among Asian A-genome Oryza species using inter simple sequence repeat (ISSR) polymorphism. Fourth International Rice Genetics Symposium, 22-27 October 2000, IRRI, Philippines. Abstracts: 212.

Salimath SS, de Oliveira AC, Godwin ID, Bennetzen JL. Assessment of genome origins and genetic diversity in the genus Eleusine with DNA markers. Genome. 1995; 38(4): 757-763.

Charters YM, Robertson A, Wilkinson MJ, Ramsay G. PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5’-anchored simple sequence repeat (SSR) primers. Theor Appl Genet. 1996; 92(3-4): 442-447.

Charters YM, Wilkinson MJ. The use of self-pollinated progenies as ‘in-groups’ for the genetic characterization of cocoa germplasm. Theor Appl Genet. 2000; 100(1): 160-166.

Tsumura Y, Ohba K, Strauss SH. Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica). Theor Appl Genet. 1996; 92(1): 40-45.

Hantula J, Dusabenyagasani M, Hamelin RC. Random amplified microsatellites (RAMS) - a novel method for characterizing genetic variation within fungi. Eur J Path. 1996; 26(3): 159-166.

Gilbert J E, Lewis RV, Wilkinson MJ, Caligari PDS. Developing an appropriate strategy to assess genetic variability in plant germplasm collections. Theor Appl Genet. 1999; 98(6-7): 1125-1131.

Gemmill CEC, Ranker TA, Ragone D, Perlman SP, Wood KR. Conservation genetics of the endangered endemic Hawaiian genus Brighamia (Campanulaceae). Am J Bot. 1998; 85(4): 528-539.


Refbacks

  • There are currently no refbacks.