Immunopharmacological activity of flavonoids from Lemna minor (Duckweed) and determined its immunological activity

Sanjay S. Sharma, Amit Gupta, Vishakha S. Mane, Bharat Shinde


The main objective of our study is to conduct its immunopharmacological activity using flavonoids extracted from whole plant of Lemna minor (duckweed), medicinal plant in virally infected human whole blood against ovalbumin (OVA), specific protein antigen. For these studies, isolated secondary metabolite i.e. flavonoid from whole plant of duckweed and evaluated its immunopharmacological activity of flavonoid using variable concentration (i.e. 1-30 mg/ml; 50 µl) on infected human whole blood samples and determine its proliferation assay containing OVA and estimation of free haemoglobin content in blood plasma. In addition, antibody production was also estimated against OVA using Elisa method. The results of these studies showed that these flavonoids at higher doses showed immunosuppressive effect because of decline in proliferation, free haemoglobin content in the blood plasma and antibody production. Overall, this study claimed that these flavonoids from duckweed showed immunosuppressive activity against OVA.


Lemna minor; Ovalbumin; Infected; Proliferation; Haemoglobin

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