Immunopharmacological activity of flavonoids from Lemna minor (Duckweed) and determined its immunological activity

Sanjay S. Sharma, Amit Gupta, Vishakha S. Mane, Bharat Shinde

Abstract


The main objective of our study is to conduct its immunopharmacological activity using flavonoids extracted from whole plant of Lemna minor (duckweed), medicinal plant in virally infected human whole blood against ovalbumin (OVA), specific protein antigen. For these studies, isolated secondary metabolite i.e. flavonoid from whole plant of duckweed and evaluated its immunopharmacological activity of flavonoid using variable concentration (i.e. 1-30 mg/ml; 50 µl) on infected human whole blood samples and determine its proliferation assay containing OVA and estimation of free haemoglobin content in blood plasma. In addition, antibody production was also estimated against OVA using Elisa method. The results of these studies showed that these flavonoids at higher doses showed immunosuppressive effect because of decline in proliferation, free haemoglobin content in the blood plasma and antibody production. Overall, this study claimed that these flavonoids from duckweed showed immunosuppressive activity against OVA.

Keywords


Lemna minor; Ovalbumin; Infected; Proliferation; Haemoglobin

Full Text:

PDF

References


Gupta A, Khamkar PR, Chaphalkar SR. Applications and uses of active ingredients from medicinal plants. Ind J Novel Drug Deliv. 2014; 6(2): 106-111.

Pichersky E, Gang DR. Genetics and biochemistry of secondary metabolites in plants: an evolutionary perspective. Trends Plant Sci. 2000; 5: 439-445.

Croteau R, Kutchan TM, Lewis NG. Natural products (secondary metabolites). Biochem Mol Biol Plants. 2000; 24: 1250-1319.

Gupta A. Immunopharmacological activity of medicinal plants. J Clin Exp Immunol. 2016; 1(1): 1-5.

Kitajima J, Kimizuka K, Tanaka Y. Three new sesquiterpenoid glucosides of Ficus pumila fruit. Chem Pharm Bull. 2000; 48(1): 77-80.

Treitler DS, Li Z, Krystal M, Meanwell NA, Snyder SA. Evaluation of HIV-1 inhibition by stereoisomers and analogues of the sesquiterpenoid hydroquinone peyssonol A. Bioorg Med Chem Lett. 2013; 23(7): 2192-2196.

Wratten SJ, Faulkner DJ, Hirotsu K, Clardy J. Diterpenoid isocyanides from the marine sponge Hymeniacidon amphilecta. Tetrahedron Lett. 1978; 19(45): 4345-4348.

Grayer RJ, Kite GC, Abou-Zaid M, Archer LJ. The application of atmospheric pressure chemical ionisation liquid chromatography-mass spectrometry in the chemotaxonomic study of flavonoids: characterisation of flavonoids from Ocimum gratissimum var. gratissimum. Phytochem Anal. 2000; 11: 257-267.

Macdonald IO, Oludare AS, Olabiyi A. Phytotoxic and anti-microbial activities of flavonoids in Ocimum gratissimum. Life Sci. 2010; 7: 3.

Kosalec I, Bakmaz M, Pepeljnjak S, Vladimir-Knezevic S. Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharm Zagreb. 2004; 54: 65-72.

Davis SM, Drake KD, Maier KJ. Toxicity of boron to the duckweed Spirodella polyrrhiza. Chemosphere. 2002; 48: 615-620.

Driever SM, Nes EHV, Roijackers RMM. Growth limitation of Lemna minor due to high plant density. Aquat Bot. 2005; 81: 245-251.

Prasad MNV, Malek P, Waloszek A, Bojko M, Strazalka K. Physiological responses of Lemna trisulca L. (duckweed) to cadmium and copper accumulation. Plant Sci. 2001; 161: 881-889.

Axtell NR, Sternberg SPK, Claussen, K. Lead and nickel removal using Microspora and Lemna minor. Biores Technol. 2003; 89: 41-48.

Kamal M, Ghaly AE, Mahmoud N, Cote R. Phytoaccumulation of heavy metals by aquatic plants. Environ Int. 2004; 29: 1029-1039.

Gupta A, Chaphalkar SR. Cytotoxic and immunosuppressive activity of terpenoids from Prosopis spicigera, Mimusops elengi and Terminalia arjuna. Int J Univ Pharm Biosci. 2016; 5(1): 32-39.

Gupta A, Chaphalkar SR. Antidiabetic activity of Calotropis gigantea in human whole blood. J Dis Global Health. 2016; 6 (3): 107-112.

Gupta A, Chaphalkar SR. Inhibition of antigen specific T cell population using Calotropis gigantea and Terminalia arjuna. J Biol Nat. 2016; 5(1): 14-19.

Bansode T, Gupta A, Shinde B, Salalkar BK. Partial purification and antidiabetic effect of bioactive compounds isolated from medicinal plants. MicroMed. 2017; 5(1): 1-7.

Gupta A, Chaphalkar SR. Immunoadjuvant potential of Azadirachta indica against rabies, hepatitis and DPT vaccine antigen. Int J Med Pharmac Sci. 2015; 5(7): 1-5.


Refbacks

  • There are currently no refbacks.